Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes

ELife
Nadinath B NillegodaB Bukau

Abstract

Hsp70 participates in a broad spectrum of protein folding processes extending from nascent chain folding to protein disaggregation. This versatility in function is achieved through a diverse family of J-protein cochaperones that select substrates for Hsp70. Substrate selection is further tuned by transient complexation between different classes of J-proteins, which expands the range of protein aggregates targeted by metazoan Hsp70 for disaggregation. We assessed the prevalence and evolutionary conservation of J-protein complexation and cooperation in disaggregation. We find the emergence of a eukaryote-specific signature for interclass complexation of canonical J-proteins. Consistently, complexes exist in yeast and human cells, but not in bacteria, and correlate with cooperative action in disaggregation in vitro. Signature alterations exclude some J-proteins from networking, which ensures correct J-protein pairing, functional network integrity and J-protein specialization. This fundamental change in J-protein biology during the prokaryote-to-eukaryote transition allows for increased fine-tuning and broadening of Hsp70 function in eukaryotes.

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Datasets Mentioned

BETA
O75953

Methods Mentioned

BETA
protein folding
FRET
proximity ligation assay
size-exclusion chromatography
environmental stresses
Phylogenetic Discriminant Analysis
NMR
transfections
proximity ligation
PCR

Software Mentioned

Phylogenetic Discriminant Analysis ( PDA
Phylogenetic Discriminant Analysis ( PDA )
DUOLINK
PDA
PyMOL
DeepView
SDA
Model webserver
Model
RaxML

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