PMID: 8444789Mar 1, 1993Paper

Evolution of bacteriophage T7 in a growing plaque

Journal of Bacteriology
J Yin

Abstract

The emergence of mutants during the 10(9)-fold amplification of a bacteriophage was spatially resolved in a growing plaque. When wild-type phage T7 was grown on an Escherichia coli host which expressed an essential early enzyme of the phage infection cycle, the T7 RNA polymerase, mutant phage relying on this enzyme appeared in 10(8) phage replications and outgrew the wild type. Spatial resolution of the selection process was achieved by analyzing stab samples taken along a plaque radius. Different mutants were selected at different rates along different radii of the plaque, based on host range and restriction patterns of the isolates. The mutants deleted up to 11% of their genomes, including the gene for their own RNA polymerase. They gained an advantage over the wild type by replicating more efficiently, as determined by one-step growth cultures.

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Citations

Apr 1, 1996·Nature Biotechnology·J J Holland
Apr 1, 1996·Nature Biotechnology·Y Lee, J Yin
Jun 1, 2011·Chembiochem : a European Journal of Chemical Biology·Sandra SöteSusanne Brakmann
May 24, 2018·Proceedings of the National Academy of Sciences of the United States of America·Aline Tabib-SalazarSivaramesh Wigneshweraraj
Nov 30, 1994·Annals of the New York Academy of Sciences·J Yin
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Mar 30, 2018·Microbiology and Molecular Biology Reviews : MMBR·John Yin, Jacob Redovich
Jun 14, 2020·The Journal of Biological Chemistry·Josh McQuailSivaramesh Wigneshweraraj
Jan 17, 2003·Biotechnology and Bioengineering·Elizabeth E EndlerJohn Yin
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Mar 1, 2006·Biotechnology and Bioengineering·Vy LamJohn Yin
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Sep 30, 2021·Annual Review of Virology·Esteban DomingoCelia Perales

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