Evolution of cnidarian trans-defensins: Sequence, structure and exploration of chemical space

Proteins
Michela L MitchellRaymond S Norton

Abstract

Many of the small, cysteine-rich ion-channel modulatory peptides found in Cnidaria are distantly related to vertebrate defensins (of the trans-defensin superfamily). Transcriptomic and proteomic studies of the endemic Australian speckled sea anemone (Oulactis sp.) yielded homologous peptides to known defensin sequences. We extended these data using existing and custom-built hidden Markov models to extract defensin-like families from the transcriptomes of seven endemic Australian cnidarian species. Newly sequenced transcriptomes include three species of Actiniaria (true sea anemones); the speckled anemone (Oulactis sp.), Oulactis muscosa, Dofleinia cf. armata and a species of Corallimorpharia, Rhodactis sp. We analyzed these novel defensin-like sequences along with published homologues to study the evolution of their physico-chemical properties in vertebrate and invertebrate fauna. The cnidarian trans-defensins form a distinct cluster within the chemical space of the superfamily, with a unique set of motifs and biophysical properties. This cluster contains identifiable subgroups, whose distribution in chemical space also correlates with the divergent evolution of their structures. These sequences, currently restricted to cnidari...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jul 25, 2000·The Journal of Biological Chemistry·D M HooverJ Lubkowski
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Jun 25, 2004·Nucleic Acids Research·Mario StankeBurkhard Morgenstern
Dec 31, 2005·Nucleic Acids Research·James C SullivanJohn R Finnerty
May 9, 2008·Genome Research·Camilla M WhittingtonKatherine Belov
Nov 11, 2008·Protein and Peptide Letters·Andrew CampenA Keith Dunker
Nov 21, 2008·The Journal of Biological Chemistry·Sascha JungJoachim Grötzinger
Jan 5, 2010·Current Biology : CB·Kim RoelantsFranky Bossuyt
Mar 23, 2011·PLoS Biology·Hervé PhilippeDenis Baurain
May 4, 2011·The Journal of Biological Chemistry·Jeremie Vidal-DupiolGuillaume Mitta
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
May 20, 2011·Nucleic Acids Research·Robert D FinnSean R Eddy
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Mar 1, 2012·Developmental and Comparative Immunology·Shunyi Zhu, Bin Gao
Mar 24, 2012·Journal of Neurophysiology·Pin LiuBruce P Bean
Oct 9, 2013·Acta Crystallographica. Section D, Biological Crystallography·Monika A CoronadoChristian Betzel
Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta
Feb 22, 2014·Molecular Biology Reports·Diyan LiQing Zhu
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Jun 11, 2015·Bioinformatics·Felipe A SimãoEvgeny M Zdobnov
Jan 21, 2016·SpringerPlus·Thomas M A ShafeeMarilyn A Anderson
Apr 12, 2016·ACS Central Science·Christina TysoeStephen G Withers
Jun 15, 2016·Molecular Biology and Evolution·Thomas M A ShafeeMarilyn A Anderson
Aug 26, 2016·Cellular and Molecular Life Sciences : CMLS·Thomas M A ShafeeMark D Hulett
Mar 7, 2017·Nature Methods·Rob PatroCarl Kingsford
Aug 11, 2017·The FEBS Journal·Chan-Hee KimNam Gyu Park
Oct 5, 2017·Genome Biology·Andrzej ZielezinskiWojciech M Karlowski
Aug 14, 2018·Bioinformatics·Thomas Shafee, Marilyn A Anderson

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Citations

Jan 16, 2020·Toxins·Rimma S KalinaIrina N Gladkikh
May 3, 2020·Molecular Biology and Evolution·Thomas ShafeeKim Johnson
Aug 9, 2019·Evolutionary Bioinformatics Online·Yongchun ZuoGuifang Cao
Jul 1, 2020·Frontiers in Immunology·Jennifer R ShelleyJulia R Dorin
Jul 13, 2020·Biochemical Pharmacology·Gabor TajtiRaymond S Norton

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