Abstract
Gliosarcoma, a variant of glioblastoma multiforme (GBM), is a highly invasive malignant tumor. Unfortunately, this disease still marked by poor prognosis regardless of modern treatments. It is of great significance to discover specific molecular probes targeting gliosarcoma for early cancer diagnosis and therapy. Herein, we have selected a group of DNA aptamers with high affinity and selectivity against gliosarcoma cells K308 using cell-SELEX. All the dissociation constants of these aptamers against gliosarcoma cells were in the nanomolar range and aptamer WQY-9 has the highest affinity and good selectivity among them. Furthermore, truncated aptamer sequence, WQY-9-B, shows similar recognition ability to aptamer WQY-9. In addition, WQY-9-B was found to be able to bind selectively and internalize into cytoplasm of target cancer cell at 37 °C. More importantly, compared to a random sequence, aptamer WQY-9-B showed excellent recognition rate (73.3%) for tissue sections of clinical gliosarcoma samples. These data suggests that aptamer WQY-9-B has excellent potential as an effective molecular probe for gliosarcoma diagnosis.
References
Aug 30, 1990·Nature·A D Ellington, J W Szostak
May 1, 1991·Cancer·J M MeisJ S Nelson
Aug 3, 1990·Science·C Tuerk, L Gold
Feb 28, 1998·Nucleic Acids Research·J D ThompsonD G Higgins
Jul 12, 2002·Genes, Chromosomes & Cancer·Bertrand ActorRuthild G Weber
May 27, 2006·Science·Ralph Weissleder
Sep 19, 2007·Molecular & Cellular Proteomics : MCP·Prabodhika MallikaratchyWeihong Tan
Mar 14, 2008·ChemMedChem·Hui William ChenWeihong Tan
Mar 28, 2008·Journal of Proteome Research·Dihua ShangguanWeihong Tan
Jan 20, 2009·Leukemia·K SefahW H Tan
Jul 21, 2009·Journal of Neuro-oncology·Seunggu J HanAndrew T Parsa
Aug 18, 2009·The Analyst·Zilong ZhaoDihua Shangguan
Mar 3, 2012·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Geetika SinghChitra Sarkar
Jun 29, 2012·Biochimie·Agnes CibielFrédéric Ducongé
Aug 6, 2014·Molecular Therapy. Nucleic Acids·Hongguang SunYouli Zu
Dec 30, 2014·In Vitro Cellular & Developmental Biology. Animal·Pei-Sen YaoZu-Cheng Ye
Mar 4, 2015·ACS Nano·Yeh-Hsing LaoKam W Leong
May 6, 2015·PloS One·Jiehua XuWeihong Tan
Jul 15, 2015·Theranostics·Xiaoqiu WuWeihong Tan
Citations
Sep 4, 2016·Talanta·Yubin ZhouTerry W J Steele
Sep 28, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Markéta SvobodováCiara K O'Sullivan
Jun 22, 2018·Oncotarget·Ricardo L PereiraHenning Ulrich
Jul 11, 2020·Advanced Biosystems·Tao BingDihua Shangguan
May 1, 2018·The Analyst·Qiaoyi WuChaoyong Yang
Oct 9, 2020·ACS Applied Materials & Interfaces·Ningqin LinChaoyong Yang
Mar 6, 2021·Chemical Reviews·Lingling WuWeihong Tan
May 8, 2021·Analytical Chemistry·Lie LiKemin Wang
Jun 10, 2021·The Analyst·Xinhuan ChenKangdong Liu
Jun 11, 2019·Analytical Chemistry·Qiaoyi WuChaoyong Yang
Jul 3, 2018·Analytical Chemistry·Alexey V SamokhvalovBoris B Dzantiev