Evolution of endogenous hormone concentration in embryogenic cultures of carrot during early expression of somatic embryogenesis

Plant Cell Reports
V M JiménezF Bangerth

Abstract

Embryogenic callus and suspension cultures of carrot (Daucus carota L., cv. Nantaise), growing on/in medium including 1 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), were transferred to medium with or without this plant growth regulator, to impair or induce, respectively, further development of somatic embryos. The endogenous hormone levels of the cultures were determined over 7 days by means of radio-immunoassay, to characterize their evolution in the initial stages of embryo development. In general, levels of indoleacetic acid (IAA) and abscisic acid (ABA) showed only short-lived differences among treatments during this time in both types of tissue analyzed (i.e., a peak of IAA in callus cultures in the absence of 2,4-D, 48 h after medium change, and higher ABA contents 144 h after subculture of suspension cultures in the presence of 2,4-D). Gibberellins (1, 3 and 20) were detected only in suspension cultures devoid of 2,4-D, starting 24 h after subculture. Concerning the evaluated cytokinins-zeatin/zeatin riboside and N6(Delta2-isopentenyl) adenine/N6(Delta2-isopentenyl) adenosine-the most remarkable observation is that high levels of the former generally coincided with low concentrations of the latter, indicating a shift fr...Continue Reading

References

Mar 23, 2000·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·A K Sharma
Mar 23, 2000·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·K OsugaA Komamine
Mar 10, 2001·Physiologia Plantarum·Víctor M. Jiménez, Fritz Bangerth
Apr 11, 2003·Journal of Plant Physiology·Yoshihiko Tokuji, Kyoko Kuriyama
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J W Einset
Jul 1, 1979·Plant Physiology·T Fujimura, A Komamine
Oct 1, 1990·Plant Physiology·P HatzopoulosZ R Sung

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Citations

Oct 8, 2016·Journal of Separation Science·Chaozhi PanLiya Ge
Aug 26, 2009·Molecular Biology Reports·Omid Karami, Abbas Saidi

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