PMID: 9177234Jun 10, 1997Paper

Evolution of HIV-1 coreceptor usage through interactions with distinct CCR5 and CXCR4 domains

Proceedings of the National Academy of Sciences of the United States of America
Z LuR W Doms

Abstract

The chemokine receptor CXCR4 functions as a fusion coreceptor for T cell tropic and dual-tropic HIV-1 strains. To identify regions of CXCR4 that are important for coreceptor function, CXCR4-CXCR2 receptor chimeras were tested for the ability to support HIV-1 envelope (env) protein-mediated membrane fusion. Receptor chimeras containing the first and second extracellular loops of CXCR4 supported fusion by T tropic and dual-tropic HIV-1 and HIV-2 strains and binding of a monoclonal antibody to CXCR4, 12G5, that blocks CXCR4-dependent infection by some virus strains. The second extracellular loop of CXCR4 was sufficient to confer coreceptor function to CXCR2 for most virus strains tested but did not support binding of 12G5. Truncation of the CXCR4 cytoplasmic tail or mutation of a conserved DRY motif in the second intracellular loop did not affect coreceptor function, indicating that phosphorylation of the cytoplasmic tail and the DRY motif are not required for coreceptor function. The results implicate the involvement of multiple CXCR4 domains in HIV-1 coreceptor function, especially the second extracellular loop, though the structural requirements for coreceptor function were somewhat variable for different env proteins. Finally,...Continue Reading

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Citations

Mar 5, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Kate L JonesJohnson Mak
Apr 2, 2013·Antiviral Research·Aiman A Haqqani, John C Tilton
Jan 29, 2000·Journal of Immunological Methods·W A MarascoA Winkler
Apr 23, 1999·Journal of Reproductive Immunology·J A HoxieT J Matthews
May 22, 2002·Journal of Virological Methods·Alexandra BittnerBarbara S Schnierle
Aug 1, 1997·Current Opinion in Immunology·J P MooreT Dragic
Dec 19, 2002·Cytokine & Growth Factor Reviews·Amber D SteeleThomas J Rogers
Nov 17, 2011·Journal of Medicinal Chemistry·Won-Tak ChoiJing An
Jun 27, 2012·The Journal of Biological Chemistry·Lusine H DemirkhanyanGregory B Melikyan
May 15, 2001·AIDS Research and Human Retroviruses·B J DoranzR W Doms
Mar 20, 2003·AIDS Research and Human Retroviruses·Satish PillaiJacques Corbeil
Jan 31, 2002·AIDS·A Brelot, M Alizon
Sep 10, 2002·AIDS·Julio Martín-GarcíaFrancisco González-Scarano
Oct 25, 2002·Proceedings. Biological Sciences·Christel Kamp, Stefan Bornholdt
Mar 8, 2005·Immunology and Cell Biology·Andrew J SloaneGarry W Lynch
Jun 8, 1999·Annual Review of Immunology·E A BergerJ M Farber
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·Elias J Fernandez, Elias Lolis
Feb 10, 2011·Journal of Translational Medicine·Samanta A MarianiGuido Poli

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