PMID: 2510156Oct 1, 1989Paper

Evolution of immunoglobulin genes: VH families in the amphibian Xenopus

Proceedings of the National Academy of Sciences of the United States of America
E HsuF W Alt

Abstract

We have isolated multiple independent cDNA clones that represent mRNA sequences of immunoglobulin heavy chains from the spleen of adult Xenopus laevis. These cDNA clones contained constant (C) region sequences that were either Cmu or a separate C region sequence believed to be Cnu. In individual cDNA clones the C region sequences were associated with independent heavy-chain variable region (VH) sequences that were classifiable into five distinct families. Genomic Southern blotting analyses with family-specific probes indicated that the haploid genome contains a minimum of 80 VH gene segments, a number similar to that found in various mammalian species. Multiple JH and putative DH segments were also identified (J, joining; D, diversity). Analyses of 13 independent VHDJH junctions suggest that combinatorial and junctional diversification mechanisms probably arose early in vertebrate evolution. Finally, comparison of Xenopus VH sequences to those from other vertebrates indicated conservation of V region framework residues that are responsible for the tertiary structure of the Fv throughout evolution.

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Citations

Jan 1, 1992·Immunogenetics·T C Tobin-Janzen, J E Womack
Feb 10, 1994·Journal of Immunological Methods·S Desravines, E Hsu
Sep 1, 1991·Molecular Immunology·E HsuL Du Pasquier
Jun 11, 1995·Nucleic Acids Research·B YangM S Coleman
Jun 8, 1999·Annual Review of Immunology·G W LitmanJ P Rast
Jul 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuko Ohta, Martin Flajnik
Mar 1, 2012·Developmental and Comparative Immunology·Yi SunYaofeng Zhao
Oct 1, 1998·European Journal of Immunology·R MussmannL Du Pasquier
Dec 1, 1996·European Journal of Immunology·R MussmannE Hsu
May 1, 1996·Thorax·F K StevensonS T Holgate
Aug 1, 1993·European Journal of Immunology·S E StewartL A Steiner

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