Evolution of insulin-like growth factor (IGF) function: production and characterization of recombinant hagfish IGF

General and Comparative Endocrinology
Z UptonF J Ballard

Abstract

While there is considerable structural evidence that insulin-like growth factors (IGFs) share a long evolutionary history, little is known about the conservation of IGF function. In order to address this, we have made recombinant hagfish IGF, hence allowing characterization of an IGF from a representative of the primitive vertebrate class, Agnatha. The production of recombinant hagfish IGF has been complicated by a number of factors including the requirement of a longer leader peptide for fusion protein expression, reduced solubility of the protein, as well as problems in the refolding procedure. However, we were able to produce a small quantity of hagfish IGF with an N-terminal glycine addition which is biologically active. Furthermore, N-terminal amino acid sequencing and mass spectrometry confirm that we have produced hagfish IGF. In vitro assessment of recombinant hagfish IGF in cultured cells indicates that hagfish IGF indeed shares functional properties with mammalian IGFs. Thus, hagfish IGF stimulates protein synthesis in rat myoblasts, but 20- and 5-fold more peptide, respectively, is required to achieve the same half-maximal responses as with human IGF-I (hIGF-I) or IGF-II (hIGF-II). Hagfish IGF also competes for bindi...Continue Reading

Citations

Sep 19, 2000·Bioscience, Biotechnology, and Biochemistry·S MoriyamaH Kawauchi
Aug 24, 2006·Bioscience, Biotechnology, and Biochemistry·Xi-Lian HuAn-Ju Zhang
Sep 8, 2015·General and Comparative Endocrinology·Genki YamagishiMin Kyun Park
May 30, 2008·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Phillip V Gordon, Marek Marcinkiewicz
May 29, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Munetaka ShimizuBrian R Beckman
Feb 11, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Z UptonF J Ballard

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