Evolution of intestinal microbiome in a process of faecal microbiota transfer (FMT) in a patient with Clostridioides difficile infection: NGS analysis with different software programs.

Enfermedades infecciosas y microbiología clínica
María Paz VenteroJuan Carlos Rodríguez

Abstract

Clostridioides difficile infection (CDI) has become a global healthcare challenge due to increases in its incidence and mortality rates. Faecal microbiota transfer (FMT) is postulated as a protocol to prevent CDI recurrence. A donor faecal sample and patient faecal samples (pre-FMT and post-FMT) were analysed. The r16S gene was amplified and sequenced by NGS, and its diversity and taxonomy composition were examined. Microbial richness increased in post-FMT samples, and the β diversity studies grouped the samples into two clusters. One included the non-pathological samples (donor and pre-FMT samples), and the other included the pathological sample. The results obtained by Qiime2 and Bioconductor were similar. The analysis showed an increase in taxonomic diversity after the FMT, which suggests its usefulness. Moreover, these results showed that standardisation of bioinformatics analysis is key.

References

Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Aug 25, 2015·World Journal of Gastrointestinal Pathophysiology·Stephen Malnick, Ehud Melzer
Feb 13, 2018·European Journal of Internal Medicine·Michael SamarkosOlga Kampouropoulou
Jul 26, 2019·Nature Biotechnology·Evan BolyenJ Gregory Caporaso

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Citations

Feb 26, 2021·Enfermedades infecciosas y microbiología clínica·Manuel Ponce-AlonsoRosa Del Campo

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