Evolution of ischemia and neovascularization in a murine model of full thickness human wound healing.

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Aos S KarimAngela Lf Gibson

Abstract

Translation of wound healing research is limited by the lack of an appropriate animal model, due to the anatomic and wound healing differences in animals and humans. Here, we characterize healing of grafted, full-thickness human skin in an in vivo model of wound healing. Full-thickness human skin, obtained from reconstructive operations, was grafted onto the dorsal flank of NOD.Cg-KitW41J Tyr + Prkdcscid Il2rgtm1Wjl /ThomJ mice. The xenografts were harvested 1 to 12 weeks after grafting, and histologic analyses were completed for viability, neovascularization, and hypoxia. Visual inspection of the xenograft shows drying and sloughing of the epidermis starting at week four. By week 12, the xenograft appears healed but has lost 63.05 ± 0.24% of the initial graft size. There is histologic evidence of epidermolysis as early as 2 weeks, which progresses until week 4, when new epidermis appears from the wound edges. Epidermal regeneration is complete by week 12, although the epidermis appears hypertrophied. An initial increase of infiltrating immune mouse cells into the xenograft normalizes to baseline 6 months after grafting. Neovascularization, as evidenced by positive staining for the proteins human CD31 and alpha smooth muscle ac...Continue Reading

References

Jan 1, 1992·World Journal of Surgery·D HeimbachJ Marvin
Mar 1, 1988·The Journal of Experimental Medicine·G C BosmaM J Bosma
Jan 30, 1999·The Journal of Pathology·M P AndriessenJ Schalkwijk
Jan 5, 2002·Carcinogenesis·Carola BerkingMeenhard Herlyn
Nov 26, 2003·Burns : Journal of the International Society for Burn Injuries·Carlotta CastagnoliGilberto Magliacani
Jan 25, 2005·The British Journal of Ophthalmology·I LeibovitchD Selva
Apr 19, 2007·Plastic and Reconstructive Surgery·Christopher S J DunkinD Angus McGrouther
Jan 21, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Chandan K Sen
Jul 21, 2010·Archives of Otolaryngology--head & Neck Surgery·Emre VuralMartin Hauer-Jensen
Aug 4, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Thomas A Mustoe, Anandev Gurjala
Apr 25, 2012·Virchows Archiv : an International Journal of Pathology·Juha P VäyrynenMarkus J Mäkinen
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Nov 20, 2012·The American Journal of Pathology·Laure RittiéGary J Fisher
Jan 8, 2013·Annals of Surgical Innovation and Research·Geoffrey C GurtnerMichael R Zenn
Sep 14, 2013·Methods in Molecular Biology·Olivera Stojadinovic, Marjana Tomic-Canic
Oct 1, 2013·BioMed Research International·Bommie F SeoSung-No Jung
Jan 1, 2014·International Wound Journal·Victoria K ShanmugamAnton Wellstein
Dec 1, 2012·Advances in Wound Care·Diego M CastillaOmaida C Velazquez
Jul 18, 2014·Advances in Wound Care·Irena PastarMarjana Tomic-Canic
Aug 3, 2014·The Surgical Clinics of North America·Edward E TredgetMatthias B Donelan
Aug 21, 2014·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jie DingEdward E Tredget
Oct 14, 2014·PloS One·Andrés A MaldonadoJulia Buján
Dec 30, 2014·Analytical Biochemistry·Ivan V Grishagin
Jan 13, 2015·Annals of Surgery·Todd W CostantiniBrian P Eliceiri
Oct 12, 2015·Immunology and Allergy Clinics of North America·Nikita Raje, Chitra Dinakar
Mar 18, 2016·Immunity·Thomas A Wynn, Kevin M Vannella
May 14, 2016·Chimerism·Brian E McIntosh, Matthew E Brown
Jul 2, 2016·Advances in Wound Care·Saad M AlrobaieaEdward E Tredget
Feb 25, 2017·Plastic and Reconstructive Surgery·Tatiana V BoykoGeorge P Yang
Jul 10, 2017·The Journal of Surgical Research·Angela L F Gibson, Shalini Shatadal
Aug 25, 2017·Methods in Molecular Biology·Jie Ding, Edward E Tredget
Feb 13, 2018·Frontiers in Immunology·Nicholas A Zumwalde, Jenny E Gumperz
Mar 27, 2018·Stem Cell Reports·Matthew E BrownWilliam J Burlingham

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Citations

May 4, 2021·Burns : Journal of the International Society for Burn Injuries·Angela GibsonKevin Eliceiri

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