Evolution of neuronal and endothelial transcriptomes in primates.

Genome Biology and Evolution
Thomas GigerSvante Pääbo

Abstract

The study of gene expression evolution in vertebrates has hitherto focused on the analysis of transcriptomes in tissues of different species. However, because a tissue is made up of different cell types, and cell types differ with respect to their transcriptomes, the analysis of tissues offers a composite picture of transcriptome evolution. The isolation of individual cells from tissue sections opens up the opportunity to study gene expression evolution at the cell type level. We have stained neurons and endothelial cells in human brains by antibodies against cell type-specific marker proteins, isolated the cells using laser capture microdissection, and identified genes preferentially expressed in the two cell types. We analyze these two classes of genes with respect to their expression in 62 different human tissues, with respect to their expression in 44 human "postmortem" brains from different developmental stages and with respect to between-species brain expression differences. We find that genes preferentially expressed in neurons differ less across tissues and developmental stages than genes preferentially expressed in endothelial cells. We also observe less expression differences within primate species for neuronal transc...Continue Reading

References

Nov 8, 1996·Science·M R Emmert-BuckL A Liotta
Jan 23, 1999·The American Journal of Pathology·F FendM Raffeld
Apr 16, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Virginie CopoisNathalie Boulle
Nov 5, 2003·Molecular Biology and Evolution·Liqing Zhang, Wen-Hsiung Li
Jan 7, 2004·Genome Research·Eitan E WinterChris P Ponting
Apr 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Andrew I SuJohn B Hogenesch
Aug 4, 2004·Genome Research·Philipp KhaitovichSvante Pääbo
Aug 30, 2005·BMC Bioinformatics·Stanislav O ZakharkinGrier P Page
Mar 11, 2006·BioTechniques·Leslie CopeRafael A Irizarry
Apr 19, 2006·Current Biology : CB·Philipp KhaitovichSvante Pääbo
Aug 22, 2006·Nature Reviews. Genetics·Philipp KhaitovichSvante Pääbo
Dec 30, 2006·PLoS Biology·Hurng-Yi WangC-K James Shen
Aug 16, 2008·PLoS Genetics·Daniel J GaffneyJacek Majewski
Sep 20, 2008·Nature Reviews. Genetics·Ajit VarkiEvan E Eichler
Sep 25, 2008·Genome Biology·Tamir TullerEytan Ruppin
Mar 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Mehmet SomelPhilipp Khaitovich
Jun 10, 2009·Current Neuropharmacology·Svetlana M StamatovicAnuska V Andjelkovic

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Citations

Feb 26, 2011·Reproductive Biology and Endocrinology : RB&E·Anna J KorzekwaDariusz J Skarzynski
Nov 22, 2011·PLoS Genetics·Dong-Dong WuYa-Ping Zhang
Oct 10, 2014·Nature Reviews. Genetics·Anamaria Necsulea, Henrik Kaessmann
Mar 5, 2013·Molecular Phylogenetics and Evolution·Dong-Dong Wu, Ya-Ping Zhang
Sep 19, 2014·Current Opinion in Genetics & Development·Mehmet SomelXiling Liu
Nov 22, 2014·Frontiers in Neuroscience·Melanie A HuntleyRyan J Watts
Jun 23, 2020·Current Opinion in Genetics & Development·Jordan A AndersonJenny Tung

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Datasets Mentioned

BETA
GSE12293

Methods Mentioned

BETA
laser capture microdissection
fluorescence-activated cell sorting
in
Assay
chips
chip

Software Mentioned

R
R Development Core Team
Ensembl
GeneChip
hyper
Func

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