Evolution of recognition of ligands from Gram-positive bacteria: similarities and differences in the TLR2-mediated response between mammalian vertebrates and teleost fish

The Journal of Immunology : Official Journal of the American Association of Immunologists
Carla M S RibeiroGeert F Wiegertjes

Abstract

We investigated the role of the TLR2 receptor in the recognition of ligands from Gram-positive bacteria in fish. Comparative sequence analysis showed a highly conserved Toll/IL-1 receptor domain. Although the leucine-rich repeat domain was less conserved, the position of the critical peptidoglycan (PGN)-binding residues in the leucine-rich repeat domain of carp TLR2 were conserved. Transfection of human embryonic kidney 293 cells with TLR2 corroborated the ability of carp TLR2 to bind the prototypical mammalian vertebrate TLR2 ligands lipoteichoic acid (LTA) and PGN from Staphylococcus aureus. The synthethic triacylated lipopeptide N-palmitoyl-S-(2,3-bis(palmitoyloxy)-(2RS)-propyl)-(R)-Cys-(S)-Ser-(S)-Lys(4) trihydrochloride (Pam(3)CSK(4)) but not the diacylated lipopeptide macrophage-activating lipopeptide-2 (MALP-2) also activated TLR2 transfected human cells. We identified clear differences between the mammalian vertebrates and carp TLR2-mediated response. The use of the same ligands on carp macrophages indicated that fish cells require high concentrations of ligands from Gram-positive bacteria (LTA, PGN) for activation and signal transduction, react less strongly (Pam(3)CSK(4)) or do not react at all (MALP-2). Overexpressio...Continue Reading

References

Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Jan 1, 1994·Methods in Enzymology·R S Rosenthal, R Dziarski
Sep 20, 1996·The Journal of Biological Chemistry·D GuptaR Dziarski
Jun 11, 1999·The Journal of Biological Chemistry·R SchwandnerC J Kirschning
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M MuzioA Mantovani
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T MatsuguchiY Yoshikai
Feb 7, 2001·The Journal of Experimental Medicine·S MorathT Hartung
May 18, 2001·Developmental and Comparative Immunology·J L StaffordM Belosevic
Jun 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M A CamposR T Gazzinelli
Jun 30, 2001·International Immunology·O TakeuchiS Akira
Mar 22, 2002·Nature Reviews. Immunology·R Medzhitov
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Veit HornungGunther Hartmann
May 3, 2002·The Journal of Biological Chemistry·Daisuke IwakiYoshio Kuroki
May 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Viola HaehnelMichael Rehli
Jun 19, 2002·The Journal of Experimental Medicine·Siegfried MorathThomas Hartung
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Osamu TakeuchiShizuo Akira
Jul 23, 2002·Trends in Immunology·Michael Rehli
Jan 16, 2003·The Journal of Biological Chemistry·Stephen E GirardinPhilippe J Sansonetti
Mar 13, 2003·Developmental and Comparative Immunology·Morgan B FarnellMichael H Kogut
Apr 15, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Stéphane WalrandMarie-Paule Vasson
Apr 17, 2003·Journal of Endotoxin Research·Kiyoshi TakedaShizuo Akira
Oct 8, 2003·Nucleic Acids Research·Ales TichopadMichael W Pfaffl
Dec 23, 2003·Molecular Immunology·Cyril JaultJohanna Chluba
Dec 23, 2003·Molecular Immunology·Annemarie H MeijerHerman P Spaink
Jun 3, 2004·The Journal of Biological Chemistry·Alexander N R WeberNicholas J Gay
Oct 21, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marisa VulcanoSilvano Sozzani
Dec 9, 2004·International Immunology·Kiyoshi Takeda, Shizuo Akira
Jun 7, 2005·Developmental and Comparative Immunology·Geert F WiegertjesJörn P Scharsack
Jun 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jared C RoachAlan Aderem
Jul 26, 2005·Infection and Immunity·Roman Dziarski, Dipika Gupta
Nov 22, 2005·FEBS Letters·Dimitar B IlievFrederick W Goetz

❮ Previous
Next ❯

Citations

Oct 5, 2010·PloS One·Carla M S RibeiroGeert F Wiegertjes
Jul 20, 2012·Advances in Hematology·Michiel van der VaartAnnemarie H Meijer
Mar 1, 2014·Inflammatory Bowel Diseases·Ye YangChristian Jobin
Jan 30, 2020·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·Wangjie CaoQiang Chen
May 24, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nazeem Shamsuddin, Ashok Kumar
Sep 16, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·M Carla PiazzonMaria Forlenza
May 14, 2019·Frontiers in Immunology·Jassy Mary S LazarteTae Sung Jung
Nov 25, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·M Carla PiazzonMaria Forlenza
Aug 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yi-feng GuJian-zhong Shao
Mar 26, 2021·Frontiers in Microbiology·Carla CuginiDaniel H Fine
Apr 2, 2011·Developmental and Comparative Immunology·Sebastian BoltañaSimon A Mackenzie

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.