Evolution of sialic acid-binding proteins: molecular cloning and expression of fish siglec-4

Glycobiology
Friederike LehmannFrank Dietz

Abstract

Siglecs are the largest family of sialic acid-recognizing lectins identified so far with 11 members in the human genome. Most of these siglecs are exclusively expressed by cells of the immune system. Comparison of different mammalian species has revealed differential and complex evolutionary paths for this protein family, even within the primate lineage. To understand the evolution of siglecs, in particular the origin of this family, we investigated the occurrence of corresponding genes in bony fish. Interestingly, only unambiguous orthologs of mammalian siglec-4, a cell adhesion molecule expressed exclusively in the nervous system, could be identified in the genomes of fugu and zebrafish, whereas no obvious orthologs of the other mammalian siglecs were found. As in mammals, fish siglec-4 expression is restricted to nervous tissues as demonstrated by northern blot. Expressed as recombinant protein, fish siglec-4 binds to sialic acids with a specificity similar to the mammalian orthologs. Relatively low sequence similarities in the cytoplasmic tail as well as an additional splice variant found in fish siglec-4 suggest alternative signaling pathways compared to mammalian species. Our observations suggest that this siglec occurs a...Continue Reading

Citations

Feb 27, 2008·Journal of Molecular Neuroscience : MN·Gunnar JeserichMelanie Pfeiffer
Aug 24, 2010·The Journal of Biological Chemistry·Xiang FanRichard Steet
Mar 5, 2016·Experimental Neurology·Jeffrey P Rasmussen, Alvaro Sagasti
Jan 24, 2007·Journal of Neurochemistry·Richard H Quarles
Apr 29, 2014·Current Opinion in Neurobiology·Thomas Becker, Catherina G Becker
Jun 2, 2006·International Review of Neurobiology·Rüdiger SchweigreiterRobert M Gould
Dec 7, 2016·Nature Communications·Matti F PronkerBert J C Janssen
Apr 29, 2005·Molecular Biology and Evolution·Heike DiekmannClaudia A O Stuermer
Jul 15, 2005·Glycobiology·Ajit Varki, Takashi Angata
May 8, 2007·Glycobiology·Takashi AngataMitsuru Nakamura
Dec 26, 2018·Frontiers in Immunology·Joyce LübbersYvette van Kooyk
Aug 22, 2007·The Biochemical Journal·Marta ManzoniEugenio Monti
May 4, 2018·Regeneration·Sukla Ghosh, Subhra Prakash Hui
Aug 8, 2012·BMC Bioinformatics·Yann Christinat, Bernard M E Moret

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.