Evolution of the β-lactam-resistant Streptococcus pneumoniae PMEN3 clone over a 30 year period in Barcelona, Spain

The Journal of Antimicrobial Chemotherapy
Jordi CàmaraCarmen Ardanuy

Abstract

To analyse the epidemiology and genetic evolution of PMEN3 (Spain9V-156), a penicillin-non-susceptible clone of Streptococcus pneumoniae, causing invasive pneumococcal disease (IPD) in Barcelona during 1987-2016. WGS was performed on 46 representative isolates and the data were used to design additional molecular typing methods including partial MLST, PCR-RFLP and detection of surface-exposed proteins and prophages, to assign the remaining isolates to lineages. The isolates were also subjected to antimicrobial susceptibility testing. Two hundred and twenty-seven adult cases of IPD caused by PMEN3 were identified. PMEN3 caused mainly pneumonia (84%) and the 30 day mortality rate was 23.1%. Evidence of recombination events was found, mostly in three regions, namely the capsular operon (associated with capsular switching) and adjacent regions containing pbp2x and pbp1a, the murM gene and the pbp2b-ddl region. Some of these genetic changes generated successful new variant serotype lineages, including one of serotype 11A that is not included in the current PCV13 vaccine. Other genetic changes led to increased MICs of β-lactams. Notably, most isolates also harboured prophages coding for PblB-like proteins. Despite these adaptations, ...Continue Reading

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Citations

Oct 15, 2019·Microbial Genomics·Tamsin C M DewéNicholas J Croucher
Apr 30, 2020·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Aida González-DíazCarmen Ardanuy
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Aug 4, 2019·Journal of Clinical Medicine·Catia CillónizAlex Soriano
Oct 11, 2020·Microorganisms·Geneviève Garriss, Birgitta Henriques-Normark
Dec 1, 2020·The Journal of Antimicrobial Chemotherapy·Jordi CàmaraCarmen Ardanuy
Jan 7, 2021·Current Opinion in Pulmonary Medicine·Ignacio Martin-Loeches, Antoni Torres
Dec 7, 2021·Frontiers in Cellular and Infection Microbiology·Antonio J Martín-Galiano, Ernesto García

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