PMID: 2504650Aug 1, 1989Paper

Evolution of the caecal epithelial barrier during Clostridium difficile infection in the mouse

Gut
M HeymanJ F Desjeux

Abstract

The most striking effect of Clostridium difficile infection is its degrading of the intestinal barrier. The aim of this study is to establish whether the cellular or paracellular constituent of the barrier is the initial target of the toxins produced by C difficile. Accordingly, the caecal epithelium of C3H/He mice was challenged under three experimental conditions with the C difficile strain VPI 10463: (1) by in vivo inoculation of axenic mice, (2) by adding the toxins to ligated caeca in vivo, and (3) by adding them to the mucosal side of isolated caeca in Ussing chambers. Under all three conditions, the epithelial barrier was tested in caeca mounted in these chambers. The transepithelial potential difference (PD), electrical conductance (G), and intact and degraded Horseradish peroxidase (HRP) fluxes were used as indexes of permeability. Results were as follows: (1) In axenic mice, C difficile caused severe infection, produced toxins A and B, reduced PD, and enhanced G and intact HRP fluxes without changing degraded HRP fluxes, (2) four hours after the toxins were added to ligated caeca in vivo, PD was relatively unaltered, but G, and intact and degraded HRP fluxes increased, and (3) when toxins were added to caeca during tw...Continue Reading

References

May 20, 1978·Lancet·H E LarsonS P Borriello
Mar 18, 1978·British Medical Journal·R H GeorgeD W Burdon
Mar 1, 1986·The Journal of Infectious Diseases·K H WilsonD Lyerly
Feb 1, 1985·Infection and Immunity·D M LyerlyT D Wilkins
Sep 1, 1974·The Journal of General Physiology·J F DesjeuxP F Curran
Jun 1, 1984·Journal of Pediatric Gastroenterology and Nutrition·M HeymanJ F Desjeux
Mar 1, 1983·Infection and Immunity·C J CzuprynskiT Wilkins

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Citations

Dec 22, 1999·The Proceedings of the Nutrition Society·M Heyman
Jul 15, 2015·Journal of Agricultural and Food Chemistry·Gregory BouchaudMarie Bodinier
Apr 7, 2018·Frontiers in Microbiology·Yinhui LiuLi Tang
Feb 26, 2021·Frontiers in Molecular Biosciences·Meng Zhou, Jiang Zhao

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