Evolution of the mycobacterial SigK regulon.

Journal of Bacteriology
Frédéric VeyrierMarcel A Behr

Abstract

Previous studies have established that members of the Mycobacterium tuberculosis complex exhibit variable production of the antigenic proteins MPT70 and MPT83 due to mutations in their positive regulator, SigK (sigma factor K), and their negative regulator, RskA (regulator of sigma K). To further understand this highly specific SigK-controlled regulon, we have undertaken evolutionary studies to determine the presence of homologues of SigK-regulated genes in other organisms and to predict its transcriptional network. Evolutionary analysis indicates that the positive and negative regulators are conserved across many organisms, but that the genes under their control are variable. Moreover, the addition, loss, and movement of various genes in the mpt70/83 locus suggest that these genes are unlikely to be cotranscribed. To test predictions from sequence analysis, we have used promoter luciferase fusions and Northern blots to show that the majority of genes in this locus have their own promoters, of which a subset are SigK regulated (mpt83, dipZ, mpt70, and Rv0449c). Next, we have shown that the intracellular inducibility of mpt70 and mpt83 is a conserved property, shared between M. tuberculosis and Mycobacterium marinum. In addition...Continue Reading

References

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Citations

Feb 27, 2010·Applied Microbiology and Biotechnology·Rachel HallerRobert Rutherford
Oct 27, 2010·Infection and Immunity·Robert A KozakMarcel A Behr
Jan 22, 2013·Tuberculosis·Jocelyne M LewBruno Sobral
Dec 3, 2009·The FEBS Journal·Preeti SachdevaYogendra Singh
Jan 14, 2017·Zoonoses and Public Health·S ThirunavukkarasuR J Whittington
Nov 2, 2016·Journal of Bacteriology·Pilar DomenechMichael B Reed
Feb 18, 2016·Journal of Bacteriology·Kelly FlentieChristina L Stallings
Apr 5, 2014·Acta Crystallographica. Section D, Biological Crystallography·Jinal ShuklaB Gopal
Mar 20, 2019·Frontiers in Veterinary Science·Victor Lorente-LealBeatriz Romero
Oct 6, 2020·Frontiers in Microbiology·Frédéric J VeyrierMarcel A Behr

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