Evolution of the neuron-specific alternative splicing product of the c-src proto-oncogene

Journal of Neuroscience Research
F RaulfM Schartl

Abstract

The observation of a slower migrating form of pp60c-src in neural tissue of chicken and mouse has recently been shown to be due to an alternative transcript form of the c-src gene (Martinez et al.: Science 237:411-415, 1987; Levy et al.: Mol Cell Biol 7:4142-4145, 1987). An insertion of 18 basepairs between exons 3 and 4, presumed to be due to alternative splicing of a mini-exon, gives rise to six amino acid residues not found in the non-neuronal (termed fibroblastic) form of pp60c-src. We have addressed the question of the evolutionary origin of the c-src neuronal insert and its functional significance regarding neural-specific expression of the c-src gene. To this end we have investigated whether the c-src gene of a lower vertebrate (the teleost fish Xiphophorus) gives rise to a neural-specific transcript in an analogous manner. We could show that the fish c-src gene does encode for a "fibroblastic" and a "neuronal" form of transcript and that the neuronal transcript does indeed arise by way of alternative splicing of a mini-exon. The mini-exon is also 18 basepairs long and we could demonstrate directly that this exon lies within the intron separating exons 3 and 4. For comparative purposes we have examined whether the fish c...Continue Reading

References

Sep 1, 1976·Nucleic Acids Research·N Blin, D W Stafford
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A GoldenJ S Brugge
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C E GeeT M Roberts
Jan 1, 1986·Journal of Neuroscience Research·P F Maness
Nov 1, 1986·Molecular and Cellular Biology·L VardimonA A Moscona
Nov 1, 1987·Molecular and Cellular Biology·J Le BeauG Walter
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P F ManessK H Pfenninger
Jan 1, 1985·Annual Review of Biochemistry·T Hunter, J A Cooper
Jun 1, 1987·Molecular and Cellular Biology·R J GregoryS G Wadsworth
Jan 1, 1985·Molecular and Cellular Biology·D W FultsP F Maness
Jun 1, 1983·Molecular and Cellular Biology·P C Cotton, J S Brugge
Oct 1, 1984·Developmental Biology·M Schartl, A Barnekow
Jun 1, 1984·Molecular and Cellular Biology·A Barnekow, M Schartl
Jul 1, 1984·Developmental Biology·B T LevyP F Maness
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jun 16, 1984·Biochimica Et Biophysica Acta·L G OfverstedtJ Chattopadhyaya
Jan 1, 1982·Differentiation; Research in Biological Diversity·M Schartl, A Barnekow

Related Concepts

Brain Chemistry
DNA, Double-Stranded
DNA Transposons
Recombination Joint
Biological Evolution
Mini-Exon
Gene Amplification
Hydra Polyps
Neurons
Proto-Oncogene Proteins

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