Evolution of the Selenoproteome in Helicobacter pylori and Epsilonproteobacteria

Genome Biology and Evolution
Pietro CravediRiccardo Percudani

Abstract

By competing for the acquisition of essential nutrients, Helicobacter pylori has the unique ability to persist in the human stomach, also causing nutritional insufficiencies in the host. Although the H. pylori genome apparently encodes selenocysteine synthase (SelA, HP1513), a key pyridoxal phosphate (PLP)-dependent enzyme for the incorporation of selenium into bacterial proteins, nothing is known about the use of this essential element in protein synthesis by this pathogen. We analyzed the evolution of the complete machinery for incorporation of selenium into proteins and the selenoproteome of several H. pylori strains and related Epsilonproteobacteria. Our searches identified the presence of selenoproteins-including the previously unknown DUF466 family-in various Epsilonproteobacteria, but not in H. pylori. We found that a complete system for selenocysteine incorporation was present in the Helicobacteriaceae ancestor and has been recently lost before the split of Helicobacter acinonychis and H. pylori. Our results indicate that H. pylori, at variance with other gastric and enterohepatic Helicobacter, does not use selenocysteine in protein synthesis and does not use selenium for tRNA wobble base modification. However, selA has...Continue Reading

Citations

Aug 18, 2016·Helicobacter·Chloë De WitteFreddy Haesebrouck
Aug 18, 2016·Helicobacter·Elvire BerthenetFilipa F Vale
Nov 28, 2017·Scientific Reports·Ana Carolina M JunqueiraStephan C Schuster
Jan 12, 2017·Nucleic Acids Research·Takahito MukaiDieter Söll
Sep 1, 2017·Genome Biology and Evolution·William G MillerKen J Forbes

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Methods Mentioned

BETA
transeq

Software Mentioned

hmmsearch
custom Perl script
EMBOSS transeq program
PyMol
BLAST
R
EMBOSS package
Espript
tRNAscan
Genedoc

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