Abstract
[125I]Triiodothyronine (T3) nuclear binding was studied in hypothalamic cultures from fetal mouse grown in serum-free medium. In enriched neuronal cultures, the apparent dissociation constant of the binding does not change with time in vitro (7 X 10(-11) M), but the maximum binding capacity (MBC) doubles between day 7 and day 14 in vitro. We show here for the first time that homologous astrocyte cell cultures, devoid of neurons as checked by tetanus toxin binding, also display T3 nuclear binding, with the same affinity as neuronal cultures. However, their MBC is 3 times lower than that of neurons after a week in vitro, and increases more quickly thereafter.
References
Aug 1, 1975·FEBS Letters·B T Hill, S Whatley
Mar 9, 1979·Science·J H Oppenheimer
Dec 1, 1977·Analytical Biochemistry·G L Peterson
Aug 1, 1985·Brain Research·M BinouxA Tixier-Vidal
Jul 1, 1985·Brain Research·J Ruel, J H Dussault
Nov 1, 1985·Endocrinology·J M KolodnyJ E Silva
Sep 3, 1986·Brain Research·M LuoJ H Dussault
Apr 15, 1983·Biochemical and Biophysical Research Communications·M A HaidarP K Sarkar
Nov 1, 1983·Neuroscience·J PuymiratA Tixier-Vidal
Citations
Jun 15, 1995·Journal of Neuroscience Research·A G TrentinV Maura Neto
Oct 1, 1988·Brain Research·R GarzaJ Puymirat
May 16, 1988·Brain Research·A Faivre-BaumanA Tixier-Vidal
Mar 1, 1989·Brain Research. Developmental Brain Research·M LuoJ H Dussault
Dec 1, 1990·Brain Research. Developmental Brain Research·V HeidetJ Epelbaum
Oct 23, 1992·Brain Research. Developmental Brain Research·J PuymiratJ H Dussault
Sep 1, 1992·Progress in Neurobiology·J Puymirat
Jan 1, 1989·Neuroscience·J PuymiratJ H Dussault
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·J M LebelJ Puymirat
Nov 1, 1996·The European Journal of Neuroscience·S PaulP K Sarkar
Feb 1, 1992·General and Comparative Endocrinology·J GagnonD Bellabarba
May 1, 1992·Experimental Neurology·I Barakat-WalterB Droz
Feb 15, 1994·Journal of Neuroscience Research·F BesnardL L Sarliève
Apr 5, 2018·Endocrinology·Beatriz MorteJuan Bernal
Dec 22, 1995·The Journal of Biological Chemistry·J PuymiratJ H Dussault
Aug 7, 1999·The Journal of Biological Chemistry·R J DenverJ Puymirat
Oct 1, 1993·Glia·J M LebelJ Puymirat
Dec 1, 2009·American Journal of Botany·Tim Wing YamKenneth M Cameron
Apr 16, 1998·Journal of Neuroendocrinology·C ApertJ Epelbaum