Evolutionarily conserved promoter region containing CArG*-like elements is crucial for smooth muscle myosin heavy chain gene expression

Circulation Research
A ZilbermanM Periasamy

Abstract

In recent years, significant progress has been made toward understanding skeletal muscle development. However, the mechanisms that regulate smooth muscle development and differentiation are presently unknown. To better understand smooth muscle-specific gene expression, we have focused our studies on the smooth muscle myosin heavy chain (SMHC) gene, a highly specific marker of differentiated smooth muscle cells. The goal of the present study was to isolate and characterize the mouse SMHC gene promoter, since the mouse promoter would be particularly suited for in vivo promoter analyses in transgenic mice and would serve as a tool for targeting genes of interest into smooth muscle cells. We report here the isolation and characterization of the mouse SMHC promoter and its 5' flanking region. DNA sequence analysis of a 2.6-kb portion of the promoter identified several potential binding sites for known transcription factors. Transient transfection analysis of promoter deletion constructs in primary cultures of smooth muscle cells showed that the region between -1208 and -1050 bp is critical for maximal SMHC promoter activity. A comparison of SMHC promoter sequences from mouse, rat, and rabbit revealed the presence of a highly conserv...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 11, 1992·Nucleic Acids Research·S Faisst, S Meyer
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P BabijM Periasamy
Sep 1, 1990·Genes & Development·E N Olson
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C MulleJ P Changeux
Aug 1, 1989·European Journal of Biochemistry·A C BorrioneS Sartore
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R NagaiM Periasamy
Jan 1, 1986·Differentiation; Research in Biological Diversity·O Kocher, G Gabbiani
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Jun 2, 1995·The Journal of Biological Chemistry·J SolwayM S Parmacek
Oct 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J QianJ L Lessard
Apr 1, 1996·Trends in Cardiovascular Medicine·Y Katoh, M Periasamy

❮ Previous
Next ❯

Citations

Jun 6, 2003·Journal of Molecular and Cellular Cardiology·Joseph M Miano
Mar 14, 2007·Molecular and Cellular Biology·Katrin DrillerAlbrecht E Sippel
Jul 1, 2004·Molecular and Cellular Biology·Markella PonticosGeorge Bou-Gharios
Feb 26, 2003·American Journal of Respiratory Cell and Molecular Biology·Hong Wei LiuJulian Solway
Feb 10, 2011·Journal of Biomedical Science·Xiaomin ZhangJeanne Y Wei
Jul 28, 2011·Gene Regulation and Systems Biology·Xiaomin ZhangJeanne Y Wei
Apr 7, 2009·Trends in Cardiovascular Medicine·Guido KrenningMartin C Harmsen
May 20, 2008·Biomaterials·Simon C Baker, Jennifer Southgate
Oct 21, 2017·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·B P HerringJ M Wo
Mar 30, 2001·The Journal of Biological Chemistry·B P HerringA M Hoggatt
Jun 28, 2005·The Journal of Biological Chemistry·Rebecca A DeatonStephen R Grant
Sep 9, 2006·The Journal of Biological Chemistry·Stéphanie PasquetNadine Thézé
Mar 29, 2000·The Journal of Biological Chemistry·J M MianoR P Misra
Jul 15, 2000·The Journal of Biological Chemistry·A M HoggattB P Herring

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.