Apr 6, 2020

Quantifying relative virulence: When μmax fails and AUC alone just isn't enough

BioRxiv : the Preprint Server for Biology
Ruben Michael Ceballos, C. L. Stacy

Abstract

One of the more challenging aspects in quantitative virology is quantifying relative virulence between two (or more) viruses that have different replication dynamics in a given susceptible host. Host growth curve analysis is often used to detail virus-host interactions and to determine the impact of viral infection on a host. Quantifying relative virulence using canonical parameters such as maximum specific growth rate (max) can fail to provide accurate information regarding experimental infection, especially for non-lytic viruses. Although area-under-the-curve (AUC) can be more robust through calculation of a percent inhibition (PIAUC), this metric can be sensitive to limit selection. In this study, using empirical and extrapolated data from Sulfolobus Spindle-shaped Virus (SSV) infections, we introduce a novel, simple metric that is proven to be more robust and less sensitive than traditional measures for determining relative virulence. This metric (ISC) more accurately aligns biological phenomena with quantified metrics from growth curve analysis to determine trends in relative virulence. It also addresses a major gap in virology by allowing comparisons between non-lytic single-virus/single-host (SVSH) infections and between...Continue Reading

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Genome
Genes
RNA, Untranslated
Genomic Stability
Cessation of Life
Gene Dosage Compensation Mechanism
Drosophila
Mnt protein, mouse
X Chromosome
Site

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

[Osaka Daigaku shigaku zasshi] The journal of Osaka University Dental Society
M Sakuda
PLoS Pathogens
Nafisa-Katrin Seich Al BasatenaBecca Asquith
Theoretical Population Biology
Thomas CaracoBoleslaw K Szymanski
© 2020 Meta ULC. All rights reserved