Microbial cells have been widely used in the industry to obtain various biochemical products, and evolutionary engineering is a common method in biological research to improve their traits, such as high environmental tolerance and improvement of product yield. To obtain better integrate functions of microbial cells, evolutionary engineering combined with other biotechnologies have attracted more attention in recent years. Classical laboratory evolution has been proven effective to letting more beneficial mutations occur in different genes but also has some inherent limitations such as a long evolutionary period and uncontrolled mutation frequencies. However, recent studies showed that some new strategies may gradually overcome these limitations. In this review, we summarize the evolutionary strategies commonly used in industrial microorganisms and discuss the combination of evolutionary engineering with other biotechnologies such as systems biology and inverse metabolic engineering. Finally, we prospect the importance and application prospect of evolutionary engineering as a powerful tool especially in optimization of industrial microbial cell factories.
Use of a glycerol-limited, long-term chemostat for isolation of Escherichia coli mutants with improved physiological properties.
Phenotypic alteration of eukaryotic cells using randomized libraries of artificial transcription factors
Prolonged selection in aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae causes a partial loss of glycolytic capacity
Parallel adaptive evolution cultures of Escherichia coli lead to convergent growth phenotypes with different gene expression states
Introducing glutathione biosynthetic capability into Lactococcus lactis subsp. cremoris NZ9000 improves the oxidative-stress resistance of the host
A high-throughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors
Genome shuffling to improve thermotolerance, ethanol tolerance and ethanol productivity of Saccharomyces cerevisiae
Micro-bioreactors for fed-batch fermentations with integrated online monitoring and microfluidic devices
Molecular characterization of clonal interference during adaptive evolution in asexual populations of Saccharomyces cerevisiae
Novel mutation breeding method for Streptomyces avermitilis using an atmospheric pressure glow discharge plasma.
Genome shuffling of Zygosaccharomyces rouxii to accelerate and enhance the flavour formation of soy sauce
Comparing the fermentation performance of Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST) and Zymomonas mobilis AX101 for cellulosic ethanol production
Adaptive evolution of nontransgenic Escherichia coli KC01 for improved ethanol tolerance and homoethanol fermentation from xylose
Batch and continuous culture-based selection strategies for acetic acid tolerance in xylose-fermenting Saccharomyces cerevisiae
Improving ethanol fermentation performance of Saccharomyces cerevisiae in very high-gravity fermentation through chemical mutagenesis and meiotic recombination
A combined physiological and proteomic approach to reveal lactic-acid-induced alterations in Lactobacillus casei Zhang and its mutant with enhanced lactic acid tolerance
The new pLAI (lux regulon based auto-inducible) expression system for recombinant protein production in Escherichia coli
Adaptive evolution of the lactose utilization network in experimentally evolved populations of Escherichia coli
Selection of a Bifidobacterium animalis subsp. lactis strain with a decreased ability to produce acetic acid
Visualizing evolution in real time to determine the molecular mechanisms of n-butanol tolerance in Escherichia coli
Systems metabolic engineering: the creation of microbial cell factories by rational metabolic design and evolution
Engineering and adaptive evolution of Escherichia coli for D-lactate fermentation reveals GatC as a xylose transporter
Xylose isomerase overexpression along with engineering of the pentose phosphate pathway and evolutionary engineering enable rapid xylose utilization and ethanol production by Saccharomyces cerevisiae
Yeast adapts to a changing stressful environment by evolving cross-protection and anticipatory gene regulation
Evolutionary Approaches for Engineering Industrially Relevant Phenotypes in Bacterial Cell Factories
Systemic understanding of Lactococcus lactis response to acid stress using transcriptomics approaches
Comparative metabolomic analysis reveals different evolutionary mechanisms for branched-chain amino acids production
Stress tolerance phenotype of industrial yeast: industrial cases, cellular changes, and improvement strategies
Time-course transcriptome analysis reveals the mechanisms of Burkholderia sp. adaptation to high phenol concentrations.
Enhancement of Acid Protease Activity of Aspergillus oryzae Using Atmospheric and Room Temperature Plasma
Improved acid-stress tolerance of Lactococcus lactis NZ9000 and Escherichia coli BL21 by overexpression of the anti-acid component recT
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