Evolutionary engineering strategies to enhance tolerance of xylose utilizing recombinant yeast to inhibitors derived from spruce biomass.

Biotechnology for Biofuels
Rakesh KoppramLisbeth Olsson

Abstract

One of the crucial factors for a sustainable and economical production of lignocellulosic based bioethanol is the availability of a robust fermenting microorganism with high tolerance to inhibitors generated during the pretreatment of lignocellulosic raw materials, since these inhibitors are known to severely hinder growth and fermentation. A long-term adaptation in repetitive batch cultures in shake flasks using a cocktail of 12 different inhibitors and a long-term chemostat adaptation using spruce hydrolysate were used as evolutionary engineering strategies to improve the inhibitor tolerance in the metabolically engineered xylose utilizing Saccharomyces cerevisiae strain, TMB3400. The yeast was evolved for a period of 429 and 97 generations in repetitive batch cultures and chemostat cultivation, respectively. During the evolutionary engineering in repetitive batch cultures the maximum specific growth rate increased from 0.18 h-1 to 0.33 h-1 and the time of lag phase was decreased from 48 h to 24 h. In the chemostat adaptation, after 97 generations, the specific conversion rates of HMF and furfural were found to be 3.5 and 4 folds higher respectively, compared to rates after three generations. Two evolved strains (RK60-5, RKU9...Continue Reading

References

Sep 1, 1994·Biotechnology Progress·M von SiversB Hahn-Hägerdal
Apr 7, 1995·Journal of Theoretical Biology·O G Berg
Nov 11, 1999·Trends in Biotechnology·A E WhealsH V Amorim
Aug 5, 2000·Applied Microbiology and Biotechnology·M J TaherzadehG Lidén
Jun 13, 2002·Bioresource Technology·Ye Sun, Jiayang Cheng
May 27, 2005·Applied Biochemistry and Biotechnology·Z Lewis LiuSteve W Gorsich
Oct 20, 2005·Journal of Bioscience and Bioengineering·M J TaherzadehG Lidén
Dec 8, 2005·Applied and Environmental Microbiology·Anneli NilssonGunnar Lidén
Feb 13, 2007·Applied Microbiology and Biotechnology·Bärbel Hahn-HägerdalMarie F Gorwa-Grauslund
Apr 1, 2008·The New Phytologist·Leonardo D GomezSimon J McQueen-Mason
Apr 29, 2008·Biotechnology and Bioengineering·Tobias ModigGunnar Lidén

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Citations

Jul 3, 2013·Microbial Cell Factories·Martin Dragosits, Diethard Mattanovich
Apr 5, 2013·Biotechnology for Biofuels·Daniel González-RamosJean-Marc G Daran
Aug 31, 2013·Chemical Reviews·Adrie J J Straathof
Oct 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bart OudJean-Marc Daran
Feb 18, 2016·Bioprocess and Biosystems Engineering·Nilesh Kumar SharmaSachin Kumar
Feb 11, 2014·Critical Reviews in Biotechnology·Antonio D MorenoMercedes Ballesteros
Dec 25, 2012·Journal of Biotechnology·Borbála ErdeiGuido Zacchi
Dec 3, 2014·Current Opinion in Biotechnology·Riaan den HaanWillem H van Zyl
Apr 20, 2014·Current Opinion in Biotechnology·Hua LingMatthew Wook Chang
Jun 19, 2013·Molecular Systems Biology·Jeffrey M SkerkerAdam P Arkin
Oct 21, 2015·Journal of Bioscience and Bioengineering·Rajni DubeyNaseem A Gaur
Dec 20, 2014·Biotechnology Letters·Violeta Sànchez Nogué, Kaisa Karhumaa
Nov 22, 2014·Journal of Industrial Microbiology & Biotechnology·Stefan RuytersBart Lievens
May 21, 2016·Frontiers in Microbiology·George E AnasontzisPaul Christakopoulos
Jul 30, 2016·Applied Microbiology and Biotechnology·Steffi A DavisonWillem Heber van Zyl
Jun 7, 2017·FEMS Yeast Research·Quinten DeparisJohan M Thevelein
Jan 1, 2012·Metabolites·Robert A Dromms, Mark P Styczynski
Feb 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daehwan Kim
Aug 31, 2014·Applied and Environmental Microbiology·Johan O WestmanCarl Johan Franzén

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Methods Mentioned

BETA
chips
protein assay

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