Evolutionary restoration of fertility in an interspecies hybrid yeast, by whole-genome duplication after a failed mating-type switch

PLoS Biology
Raúl A Ortiz-MerinoKenneth H Wolfe

Abstract

Many interspecies hybrids have been discovered in yeasts, but most of these hybrids are asexual and can replicate only mitotically. Whole-genome duplication has been proposed as a mechanism by which interspecies hybrids can regain fertility, restoring their ability to perform meiosis and sporulate. Here, we show that this process occurred naturally during the evolution of Zygosaccharomyces parabailii, an interspecies hybrid that was formed by mating between 2 parents that differed by 7% in genome sequence and by many interchromosomal rearrangements. Surprisingly, Z. parabailii has a full sexual cycle and is genetically haploid. It goes through mating-type switching and autodiploidization, followed by immediate sporulation. We identified the key evolutionary event that enabled Z. parabailii to regain fertility, which was breakage of 1 of the 2 homeologous copies of the mating-type (MAT) locus in the hybrid, resulting in a chromosomal rearrangement and irreparable damage to 1 MAT locus. This rearrangement was caused by HO endonuclease, which normally functions in mating-type switching. With 1 copy of MAT inactivated, the interspecies hybrid now behaves as a haploid. Our results provide the first demonstration that MAT locus damag...Continue Reading

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Citations

May 31, 2017·Yeast·Nurzhan KuanyshevPaola Branduardi
Aug 27, 2017·Applied and Environmental Microbiology·Jun WatanabeYuichiro Tsukioka
Jul 24, 2020·Journal of Bioinformatics and Computational Biology·Gatis MelkusJuris Viksna
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Jul 30, 2019·Current Biology : CB·Tadeusz KrassowskiKenneth H Wolfe
Nov 13, 2018·Cell·Xing-Xing ShenAntonis Rokas
Mar 27, 2021·Nature Reviews. Microbiology·Jan SteenselsKevin J Verstrepen
May 11, 2021·World Journal of Microbiology & Biotechnology·L Solieri

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Datasets Mentioned

BETA
ATCC60483
CP019506
CP019507

Methods Mentioned

BETA
3 hybrids
PCR
RNA-Seq
dissection
genotyping

Software Mentioned

PASA
PAML suite
Seaview
BLASTN
YGAP
SSPACE3
Trinity
CLUSTALW
Yeast Gene Order Browser
PhyML

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