Evolutionary specialization of MscCG, an MscS-like mechanosensitive channel, in amino acid transport in Corynebacterium glutamicum

Scientific Reports
Yoshitaka NakayamaB Martinac

Abstract

MscCG, a mechanosensitive channel of Corynebacterium glutamicum provides a major export mechanism for glutamate in this Gram-positive bacterium, which has for many years been used for industrial production of glutamate and other amino acids. The functional characterization of MscCG is therefore, of great significance to understand its conductive properties for different amino acids. Here we report the first successful giant spheroplast preparation of C. glutamicum amenable to the patch clamp technique, which enabled us to investigate mechanosensitive channel activities of MscCG in the native membrane of this bacterium. Single channel recordings from these spheroplasts revealed the presence of three types of mechanosensitive channels, MscCG, MscCG2, and CgMscL, which differ largely from each other in their conductance and mechanosensitivity. MscCG has a relatively small conductance of ~340 pS followed by an intermediate MscCG2 conductance of ~1.0 nS and comparably very large conductance of 3.7 nS exhibited by CgMscL. By applying Laplace's law, we determined that very moderate membrane tension of ~5.5 mN/m was required for half activation of MscCG compared to ~12 mN/m required for half activation of both MscCG2 and CgMscL. Furthe...Continue Reading

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Citations

May 6, 2019·Biophysics Reviews·Yoshitaka NakayamaBoris Martinac
Apr 9, 2020·Extremophiles : Life Under Extreme Conditions·Jasmina VandrichHans Jörg Kunte
Mar 23, 2019·Biophysics Reviews·Charles G Cranfield
Nov 28, 2020·European Biophysics Journal : EBJ·Sarah C JohnsonHannah R Malcolm
Dec 18, 2020·Biophysical Journal·Amar VelicPrasad K D V Yarlagadda
Jan 11, 2020·Biochimica Et Biophysica Acta. Biomembranes·Brittni L MillerHannah R Malcolm
Apr 23, 2021·Biophysical Journal·Miranda L JacobsNeha P Kamat
Aug 28, 2021·International Journal of Molecular Sciences·Miklós LengyelGábor Czirják

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Methods Mentioned

BETA
Transmission electron microscopy

Software Mentioned

TCL
Pymol Graphics System
NAMD
Visual Molecular Dynamics ( VMD )
pClamp
SOLVATE
CLUSTALW
Clampfit
CHARMM36

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