Evolvement of transgenic male-sterility and fertility-restoration system in rice for production of hybrid varieties

Plant Molecular Biology
Gundra Sivakrishna RaoKhareedu Venkateswara Rao

Abstract

We have developed a unique male-sterility and fertility-restoration system in rice by combining Brassica napus cysteine-protease gene (BnCysP1) with anther-specific P12 promoter of rice for facilitating production of hybrid varieties. In diverse crop plants, male-sterility has been exploited as a useful approach for production of hybrid varieties to harness the benefits of hybrid vigour. The promoter region of Os12bglu38 gene of rice has been isolated from the developing panicles and was designated as P12. The promoter was fused with gusA reporter gene and was expressed in Arabidopsis and rice systems. Transgenic plants exhibited GUS activity in tapetal cells and pollen of the developing anthers indicating anther/pollen-specific expression of the promoter. For engineering nuclear male sterility, the coding region of Brassica napus cysteine protease1 (BnCysP1) was isolated from developing seeds and fused to P12 promoter. Transgenic rice plants obtained with P12-BnCysP1 failed to produce functional pollen grains. The F1 seeds obtained from BnCysP1 male-sterile plants and untransformed controls showed 1:1 (tolerant:sensitive) ratio when germinated on the MS medium supplemented with phosphinothricin (5 mg/l), confirming that the ma...Continue Reading

References

Jun 1, 1989·Molecular & General Genetics : MGG·D TwellS McCormick
Mar 1, 1993·Molecular & General Genetics : MGG·T SchmüllingJ Schell
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·E ZabaletaA Araya
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Mar 31, 1999·The Plant Journal : for Cell and Molecular Biology·F X Xu, M L Chye
Feb 24, 2001·Plant Molecular Biology·H Fukuda
Feb 24, 2001·Plant Molecular Biology·E P BeersC Zhao
Feb 25, 2003·Journal of Experimental Botany·Simon A CoupeJocelyn R Eason
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
Aug 2, 2005·Plant Physiology·Michele L EngelSheila McCormick
Sep 23, 2006·Plant & Cell Physiology·Min-Jung HanGynheung An
Jan 2, 2007·BMC Plant Biology·Rodjana OpassiriJames R Ketudat Cairns
Feb 13, 2010·Science·Mark Tester, Peter Langridge
Dec 1, 1988·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S R McCouchS D Tanksley
Dec 10, 2013·Annual Review of Plant Biology·Letian Chen, Yao-Guang Liu
Mar 13, 2014·Functional & Integrative Genomics·Pawan ShuklaPulugurtha Bharadwaja Kirti
Jun 16, 2015·Scientific Reports·Surendra Pratap SinghSamir V Sawant
Mar 20, 2016·Plant Science : an International Journal of Experimental Plant Biology·Pawan ShuklaP B Kirti
Nov 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Zhenyi ChangXing Wang Deng
Feb 9, 2017·Plant Science : an International Journal of Experimental Plant Biology·Gundra Sivakrishna RaoKhareedu Venkateswara Rao

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Citations

Jan 18, 2019·International Journal of Environmental Research and Public Health·Qian YuLin He
Aug 14, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Pawan ShuklaPulugurtha Bharadwaja Kirti
Mar 26, 2021·Frontiers in Plant Science·Adil AbbasLiyong Cao
Sep 28, 2021·Journal of Experimental Botany·Su-Hyeon ShimJong-Seong Jeon

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Methods Mentioned

BETA
transgenic
PCR
electrophoreses
transgenics

Software Mentioned

Primer Express
BLASTn

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