Evolving applications of the egg: chorioallantoic membrane assay and ex vivo organotypic culture of materials for bone tissue engineering

Journal of Tissue Engineering
Karen M MarshallRichard Oc Oreffo

Abstract

The chick chorioallantoic membrane model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the chorioallantoic membrane assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the chorioallantoic membrane assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches, including timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo chorioallantoic membrane assay and the ex vivo organotypic culture to advance the field of bone tissue engineering, and highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.

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Citations

Jun 30, 2021·Experimental Cell Research·Domenico RibattiTiziana Annese
Jul 17, 2021·Biomedical Physics & Engineering Express·Benita S MackayBen Mills

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Methods Mentioned

BETA
xenograft
sedation
transfection
Assay
transgenic
dissection
PCR

Software Mentioned

Image J
Microfil
Angiotool
AngioQuant
ARRIVE
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