DOI: 10.1101/482273Nov 29, 2018Paper

EvoMining reveals the origin and fate of natural products biosynthetic enzymes

BioRxiv : the Preprint Server for Biology
Nelly Selem-MojicaFrancisco Barona-Gómez

Abstract

Natural products, or specialized metabolites, are important for medicine and agriculture alike, as well as for the fitness of the organisms that produce them. Microbial genome mining aims at extracting metabolic information from genomes of microbes presumed to produce these compounds. Typically, canonical enzyme sequences from known biosynthetic systems are identified after sequence similarity searches. Despite this being an efficient process the likelihood of identifying truly novel biosynthetic systems is low. To overcome this limitation we previously introduced EvoMining, a genome mining approach that incorporates evolutionary principles. Here, we release and use our latest version of EvoMining, which includes novel visualization features and customizable databases, to analyze 42 central metabolic enzyme families conserved throughout Actinobacteria, Cyanobacteria, Pseudomonas and Archaea. We found that expansion-and-recruitment profiles of these enzyme families are lineage specific, opening a new metabolic space related to shell enzymes, which have been overlooked to date. As a case study of canonical shell enzymes, we characterized the expansion and recruitment of glutamate dehydrogenase and acetolactate synthase into scyto...Continue Reading

Related Concepts

Acetolactate Synthase
Antibiotics
Case-Control Studies
Cell Growth
Pharmaceutical Preparations
Biological Evolution
Genome
Glutamate Dehydrogenase
Metabolism
Pseudomonas

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