Ex vivo analysis of human antigen-specific CD8+ T-cell responses: quality assessment of fluorescent HLA-A2 multimer and interferon-gamma ELISPOT assays for patient immune monitoring

Journal of Immunotherapy
D SpeiserP Romero

Abstract

The authors developed a standardized approach for immune monitoring of antigen-specific CD8+ T cells within peripheral blood lymphocytes (PBLs) that combines direct ex vivo analysis of Melan-A/MART-1 and influenza-specific CD8+ T cells with HLA-A2/peptide multimers and interferon-gamma ELISPOT assays. Here the authors assessed the quality of results obtained with 180 PBLs from healthy donors and melanoma patients. Reproducibility of the multimer assay was good (average of 15% variation). In the absence of in vivo antigen-specific T-cell responses, physiologic fluctuations of multimer-positive T cells was low, with variation coefficients of 20% for Melan-A and 28% for influenza-specific T cells. In contrast, patients with vaccination-induced T-cell responses had significantly increased T-cell frequencies clearly exceeding physiologic fluctuations. Comparable results were obtained with ELISPOT assays. In conclusion, this approach is well suited to assess T-cell responses as biologic endpoints in clinical vaccine studies.

References

Jan 12, 1996·Science·R M Zinkernagel
Apr 5, 1996·Science·E C Butcher, L J Picker
Jul 3, 1996·International Journal of Cancer. Journal International Du Cancer·E JägerA Knuth
Oct 4, 1996·Science·J D AltmanM M Davis
Jun 11, 1997·International Journal of Cancer. Journal International Du Cancer·C ScheibenbogenU Keilholz
Sep 2, 1998·Current Opinion in Immunology·G S Ogg, A J McMichael
Sep 22, 1998·The Journal of Experimental Medicine·G S OggV Cerundolo
May 12, 2000·Immunological Reviews·N M ValianteS Abrignani
Jul 6, 2000·The Journal of Experimental Medicine·V AppayS L Rowland-Jones
Mar 3, 2001·Current Opinion in Immunology·C YeeP D Greenberg
Mar 10, 2001·Nature·P ChampagneG Pantaleo
Mar 17, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·R M SimonJ A Berzofsky
Apr 20, 2001·Nature·A J McMichael, S L Rowland-Jones
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·L FongE G Engleman
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·P G CoulieT Boon
Jan 17, 2002·Journal of Immunological Methods·Jeffrey R CurrierJosephine H Cox
May 11, 2002·Nature Reviews. Immunology·Paul KlenermanP Rod Dunbar

❮ Previous
Next ❯

Citations

Jun 6, 2007·The Journal of Clinical Investigation·Deepa RastogiRachel L Miller
May 3, 2005·Journal of Translational Medicine·Craig L Slingluff, Daniel E Speiser
Sep 19, 2012·Cancer Immunology, Immunotherapy : CII·Satwinder Kaur SinghSjoerd H van der Burg
Aug 28, 2007·Cancer Immunology, Immunotherapy : CII·C M BrittenA Hoos
Jul 1, 2011·International Journal of Cancer. Journal International Du Cancer·Holger KrönigHelga Bernhard
Feb 8, 2005·The Journal of Clinical Investigation·Daniel E SpeiserPedro Romero
Apr 10, 2015·Cancer Immunology, Immunotherapy : CII·Cécile GouttefangeasCedrik M Britten
Feb 7, 2008·Expert Review of Vaccines·Sjoerd H van der Burg
Feb 16, 2008·Lancet·Alberto MantovaniFrancesco M Marincola
Jul 2, 2016·Cytometry. Part B, Clinical Cytometry·P Anoop ChandranCécile Gouttefangeas
Sep 16, 2010·Journal of Immunotherapy·Daniel E SpeiserMartin F Bachmann
May 12, 2007·Expert Review of Anticancer Therapy·Ferdy J LejeuneDaniel Speiser
Apr 9, 2014·The Journal of Experimental Medicine·Yinan Wang, Deepta Bhattacharya
May 2, 2008·Clinical and Vaccine Immunology : CVI·Bruce D ForrestWilliam C Gruber
May 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cédric TouvreyDaniel E Speiser
Feb 21, 2006·Cancer Research·Petra BaumgaertnerDaniel E Speiser
Feb 10, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bruce E LovelandPaul L R Mitchell
Jan 7, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Begoña Comin-AnduixAntoni Ribas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.