Ex-vivo and in vitro protective effects of kolaviron against oxygen-derived radical-induced DNA damage and oxidative stress in human lymphocytes and rat liver cells

Cell Biology and Toxicology
Ebenezer O FarombiL O Dragsted

Abstract

The present study reports the protective effects of kolaviron, a Garcinia biflavonoid from the seeds of Garcinia kola widely consumed in some West African countries against oxidative damage to molecular targets ex-vivo and in vitro. Treatment with hydrogen peroxide (H2O2) at a concentration of 100 micromol/L increased the levels of DNA strand breaks and oxidized purine (formamidopyrimidine glycosylase (FPG) and pyrimidine (endonuclease III (ENDO III) sites) bases in both human lymphocytes and rat liver cells using alkaline single cell gel electrophoresis (the comet assay). Kolaviron was protective at concentrations between 30-90 micromol/L and decreased H2O2-induced DNA strand breaks and oxidized bases. Neither alpha-tocopherol nor curcumin decreased H2O2-induced DNA damage in this assay. In lymphocytes incubated with Fe3+/GSH, Fe3+ was reduced to Fe2+ by GSH initiating a free radical generating reaction which induced 11.7, 6.3, and 4.9 fold increase respectively in strand breaks, ENDO III and FPG sensitive sites compared with control levels. Deferoxamine (2 mmol/L), an established iron chelator significantly inhibited GSH/Fe3+-induced strand breaks and oxidized base damage. Similarly, kolaviron at 30 and 90 micromol/L signific...Continue Reading

Citations

May 24, 2007·Marine Biotechnology·Rohan KarawitaYou-Jin Jeon
Jul 22, 2011·International Journal of Environmental Research and Public Health·Ebenezer O Farombi, Olatunde Owoeye
Mar 12, 2013·Environmental Toxicology and Pharmacology·I A AdedaraE O Farombi
Nov 3, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Oluwatosin A AdaramoyeE Olatunde Farombi
May 2, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xian-Jun QuShu-Xiang Cui
Mar 18, 2008·Free Radical Biology & Medicine·Marina E SolovievaVladimir S Akatov
Jan 12, 2007·Basic & Clinical Pharmacology & Toxicology·Ebenezer Olatunde FarombiMatthew O Oyeyemi
Mar 24, 2012·Journal of Natural Products·Wing Keung LiuChun-Tao Che
Apr 20, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M A HorstF M Lajolo
Feb 23, 2018·Journal of Evidence-based Integrative Medicine·Joseph Gbenga OmoleBenson Akinloye Fadeyi
Jan 1, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Mary GulumianVanessa Steenkamp

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