Ex-vivo assessment of chronic toxicity of low levels of cadmium on testicular meiotic cells

Toxicology and Applied Pharmacology
Cendrine Geoffroy-SiraudinMarie-Roberte Guichaoua

Abstract

Using a validated model of culture of rat seminiferous tubules, we assessed the effects of 0.1, 1 and 10 μg/L cadmium (Cd) on spermatogenic cells over a 2-week culture period. With concentrations of 1 and 10 μg/L in the culture medium, the Cd concentration in the cells, determined by ICP-MS, increased with concentration in the medium and the day of culture. Flow cytometric analysis enabled us to evaluate changes in the number of Sertoli cells and germ cells during the culture period. The number of Sertoli cells did not appear to be affected by Cd. By contrast, spermatogonia and meiotic cells were decreased by 1 and 10 μg/L Cd in a time and dose dependent manner. Stage distribution of the meiotic prophase I and qualitative study of the synaptonemal complexes (SC) at the pachytene stage were performed by immunocytochemistry with an anti SCP3 antibody. Cd caused a time-and-dose-dependent increase of total abnormalities, of fragmented SC and of asynapsis from concentration of 0.1 μg/L. Additionally, we observed a new SC abnormality, the "motheaten" SC. This abnormality is frequently associated with asynapsis and SC widening which increased with both the Cd concentration and the duration of exposure. This abnormality suggests that C...Continue Reading

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Citations

Aug 8, 2015·International Journal of Environmental Health Research·Yuyan LiErsheng Gao
Apr 23, 2013·Expert Opinion on Therapeutic Targets·H T WanC Yan Cheng
Apr 8, 2017·Oncotarget·Tianshu WeiJu Liu
Jun 4, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Philippe DurandMarie-Hélène Perrard

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