Ex vivo blockade of endothelin-1 inhibits graft coronary artery disease in a rodent cardiac allograft model

The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation
Atsushi YamaguchiRobert C Robbins

Abstract

Graft coronary artery disease (GCAD) is characterized by vascular narrowing resulting from intimal hyperplasia. Endothelin (ET)-1, derived from the vascular endothelium and macrophages, stimulates vascular smooth muscle cells (SMCs), which leads to neointimal formation in donor graft coronary arteries. In this study, we hypothesized that antisense (AS) oligodeoxynucleotides (ODN) for preproendothelin-1 (ppET-1) delivered to rat cardiac allografts by means of hyperbaric pressure would reduce the incidence of GCAD. PVG donor hearts were infused with ppET-1 AS ODN (80 micromol/liter), sense ODN, scrambled ODN or saline alone and incubated in a pressure chamber at 75 psi or ambient pressure for 45 minutes. Cardiac allografts were heterotopically transplanted into ACI rats treated with cyclosporine (7.5 mg/kg, Days 0 to 9). Allografts were procured at post-operative days (POD) 7 or 90. Reverse transcription-polymerase chain reaction (RT-PCR) assay for ET-1 mRNA and ET01 immunohistochemistry (IHC) were performed at PODs 7 and 90. Elastic staining and IHC with anti-macrophage and alpha-SMC actin antibodies were performed to assess GCAD at POD 90. Treatment with AS ODN and pressure significantly reduced ET-1 mRNA and protein expression...Continue Reading

References

Apr 10, 1992·European Journal of Pharmacology·E H OhlsteinC P Sung
Feb 28, 1991·Biochemical and Biophysical Research Communications·T SakuraiT Masaki
Feb 1, 1969·The Journal of Thoracic and Cardiovascular Surgery·K Ono, E S Lindsey
Mar 1, 1995·Current Opinion in Cardiology·R J Duquesnoy, A J Demetris
Jan 15, 1993·The American Journal of Cardiology·G J HaasR J Cody
Feb 1, 1996·The Journal of Thoracic and Cardiovascular Surgery·J DalesandroM D Allen
Sep 10, 1998·The Journal of Thoracic and Cardiovascular Surgery·R S PostonR C Robbins
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M J MannV J Dzau
Jul 27, 1999·The Annals of Thoracic Surgery·B T FeeleyR C Robbins

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Citations

Mar 2, 2005·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Masashi TanakaRobert C Robbins
Sep 7, 2005·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Michel HaddadWilliam J Kostuk
Mar 2, 2006·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Chihiro SuzukiEiji Kobayashi
Oct 8, 2013·Nitric Oxide : Biology and Chemistry·Jing LeiTimothy R Billiar
Feb 13, 2007·Transplant International : Official Journal of the European Society for Organ Transplantation·Marjan BoermaMartin Hauer-Jensen
Mar 22, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Michael Weis, John P Cooke
Jul 22, 2010·Pharmaceutical Biology·Mahnaz KhanaviSeyed Nasser Ostad
Oct 17, 2003·Coronary Artery Disease·Arshia Noori, Samer Kabbani

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