Ex vivo cultivation of corneal limbal epithelial cells in a thermoreversible polymer (Mebiol Gel) and their transplantation in rabbits: an animal model

Tissue Engineering. Part a
G SitalakshmiSamuel Abraham

Abstract

We evaluated the efficacy of autologous expanded corneal epithelial cell transplants derived from harvested limbal biopsy cultured on a thermoreversible polymer (Mebiol Gel) for the management of unilateral limbal stem cell deficiency (LSCD). Corneal limbal biopsies from 12 rabbits were cultured on a thermoreversible polymer Mebiol Gel at 37 degrees C. Cells were harvested from the dishes after 3 weeks by reducing temperature to 4 degrees C. Autologous transplantation was undertaken to reconstruct the experimentally induced limbal stem cell deficiency in the rabbit eyes. The corneas of both eyes of all rabbits were harvested later for molecular studies. Reparative surgery was a total success in seven rabbits, partial success in two, and failure in three eyes. Histology of the seven successful eyes showed the successful growth of the corneal epithelium. Immunohistochemistry and reverse transcriptase polymerase chain reaction showed the cornea phenotype and stem cell-associated markers in the limbus of the seven successful eyes, indicating the homing of these cells into limbus. In the three failure cases and in the two control rabbit eyes, used in the study, histology showed presence of goblet cells and vascularization in the str...Continue Reading

References

May 1, 1989·Ophthalmology·K R Kenyon, S C Tseng
Jun 3, 1999·The New England Journal of Medicine·K TsubotaJ Shimazaki
Jul 13, 2000·The New England Journal of Medicine·R J TsaiJ K Chen
Jan 9, 2001·Clinical Microbiology Reviews·R S BonevaL E Chapman
Nov 12, 2003·Survey of Ophthalmology·Martin GrueterichScheffer C G Tseng
Apr 20, 2004·Biochemical and Biophysical Research Communications·Keiichi HishikawaToshiro Fujita
Sep 17, 2004·The New England Journal of Medicine·Kohji NishidaYasuo Tano
Dec 13, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Taizo SumideYasuo Tano
Jan 25, 2006·Investigative Ophthalmology & Visual Science·Yasutaka HayashidaYasuo Tano
Apr 26, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tongguang WangAvindra Nath
Sep 22, 2006·The British Journal of Ophthalmology·Nick Di GirolamoMinas T Coroneo
Dec 13, 2006·Archives of Ophthalmology·Ivan R SchwabDamien G Harkin
Dec 26, 2006·Journal of Biomedical Materials Research. Part a·Mirko NitschkeCarsten Werner
Jan 26, 2007·Investigative Ophthalmology & Visual Science·Wei LiScheffer C G Tseng
May 1, 2007·Regenerative Medicine·Sajjad AhmadMajlinda Lako
Jul 12, 2007·Journal of Biomaterials Science. Polymer Edition·X Duan, H Sheardown
Sep 1, 2007·Biomaterials·Joseph YangTeruo Okano

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Citations

Jun 26, 2013·Expert Reviews in Molecular Medicine·Romesh I AngunawelaJulie T Daniels
Sep 19, 2009·Regenerative Medicine·Samantha Traphagen, Pamela C Yelick
Mar 27, 2015·Journal of Functional Biomaterials·Hannah J LevisJulie T Daniels
Jan 21, 2016·Stem Cells International·Michel HaagdorensNadia Zakaria
Jul 3, 2013·Journal of Biomedical Materials Research. Part a·Xianghua XiaoShengli Mi
Nov 8, 2012·Acta Biomaterialia·Ilida OrtegaFrederik Claeyssens
Aug 1, 2012·Drug Discovery Today·Bernice WrightChe J Connon
Nov 24, 2018·International Journal of Urology : Official Journal of the Japanese Urological Association·Surya P VaddiSamuel Jk Abraham
Feb 3, 2018·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Kristina SpaniolStefan Schrader
Jan 4, 2019·Tissue Engineering and Regenerative Medicine·Joan OlivaYutaka Niihara
May 31, 2019·ACS Applied Materials & Interfaces·Calogero FioricaGaetano Giammona

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Methods Mentioned

BETA
biopsy
periodic acid Schiff
biopsies
PCR

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