Ex vivo evaluation in normal dogs of insulin released by a bioartificial pancreas containing isolated rat islets of Langerhans

Artificial Organs
J LepeintreG Reach

Abstract

In bioartificial pancreatic systems, isolated islets of Langerhans are protected against immune rejection by an artificial membrane, permeable to glucose and insulin, but not to immunoglobulins and lymphocytes. Some of these devices, referred to as vascular systems, are set up to be connected to a vascular site in the recipient, with blood circulating in contact with one side of the membrane, and the islets on the other side. Such a bioartificial pancreas, containing isolated rat islets of Langerhans, was connected to an arteriovenous shunt of a normal anesthetized dog. The aim of this experiment was to investigate the kinetics of the insulin secretory response of the system to a glucose load. Glucose was infused upstream of the system, increasing the glucose concentration inside the bioartificial pancreas from 7 to 14 mmol/l, without altering the blood glucose concentration of the dog. Insulin concentration was determined simultaneously upstream and downstream of the bioartificial pancreas. Insulin production was calculated by multiplying the difference between these values by the blood flow rate. Blood flow rate (Q) was estimated from the change in the glucose concentration produced by the glucose infusion using a mass transf...Continue Reading

References

Dec 1, 1976·The Journal of Clinical Investigation·A D CherringtonW W Lacy
Feb 1, 1988·Journal of Biomechanical Engineering·M Y JaffrinD Notelet
Apr 1, 1988·Artificial Organs·F MoussyM Friedrich
Jan 1, 1987·Diabetologia·Y T KruszynskaK G Alberti

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Citations

Mar 1, 1993·Diabetic Medicine : a Journal of the British Diabetic Association·G Reach

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