Ex vivo-expanded NK cells from blood and ascites of ovarian cancer patients are cytotoxic against autologous primary ovarian cancer cells

Cancer Immunology, Immunotherapy : CII
Tina NhamAli A Ashkar

Abstract

Ovarian cancer (OC) is the leading cause of gynecological cancer-related death in North America. Most ovarian cancer patients (OCPs) experience disease recurrence after first-line surgery and chemotherapy; thus, there is a need for novel second-line treatments to improve the prognosis of OC. Although peripheral blood-derived NK cells are known for their ability to spontaneously lyse tumour cells without prior sensitization, ascites-derived NK cells (ascites-NK cells) isolated from OCPs exhibit inhibitory phenotypes, impaired cytotoxicity and may play a pro-tumourigenic role in cancer progression. Therefore, it is of interest to improve the cytotoxic effector function of impaired OCP ascites-NK cells at the tumour environment. We investigated the efficacy of using an artificial APC-based ex vivo expansion technique to generate cytotoxic, expanded NK cells from previously impaired OCP ascites-NK cells, for use in an autologous model of NK cell immunotherapy. We are the first to obtain a log-scale expansion of OCP ascites-NK cells that upregulate the surface expression of activating receptors NKG2D, NKp30, NKp44, produce robust amounts of anti-tumour cytokines in the presence of OC cells and mediate direct tumour cytotoxicity agai...Continue Reading

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Citations

Nov 1, 2018·Oncoimmunology·Jeremiah L OyerAlicja J Copik
Aug 23, 2019·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Milad MoloudizargariEsmaeil Mortaz
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Apr 10, 2020·Gynecologic Oncology·Janneke S Hoogstad-van EvertHarry Dolstra
Jun 29, 2021·Frontiers in Oncology·Faroogh MarofiMostafa Jarahian

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