Ex vivo expansion of human pancreatic endocrine cells

The Journal of Clinical Endocrinology and Metabolism
G M BeattieA Hayek

Abstract

Cell transplantation as a therapy for type 1 diabetes is facilitated by ex vivo cell expansion of pancreatic beta-cells without loss of differentiative characteristics. The aim of this study was to determine the optimal conditions for in vitro growth of functional human pancreatic endocrine tissue. We examined the mitogenicity of matrixes from a variety of cell lines; proliferation was greater in cells growing on matrixes from bladder carcinoma cell lines, especially in monolayers grown on matrix from the human cell line HTB-9. After 14-day culture, there was a more than 100-fold proliferative increase, which was augmented to a more than 200-fold when hepatocyte growth factor/scatter factor was added; however, hepatocyte growth factor/scatter factor induced a rapid decrease in insulin content. Without the growth factor, fetal cell monolayers expanded 4-fold with no insulin loss; however, after 12-fold expansion, the insulin levels decreased to 40% of those in unexpanded cells. Adult islet cells expanded 3-fold without insulin loss. After 5-fold expansion, insulin levels decreased by 25% compared to those in free floating islets while retaining a normal response to secretagogues. Together, these results indicate that HTB-9 matri...Continue Reading

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Citations

Apr 15, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Eszter PaisDonald B Kohn
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Dina Fomina-YadlinStuart L Schreiber
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·S Bonner-WeirJ J O'Neil
Mar 30, 2012·The Journal of Biological Chemistry·Yael BarShimon Efrat
Jun 18, 2004·Reproductive Biology and Endocrinology : RB&E·Linda B LesterDon P Wolf
Sep 17, 2008·The Review of Diabetic Studies : RDS·Shimon Efrat
May 30, 2009·Cell Transplantation·John C StendahlSamuel I Stupp
Dec 17, 2010·Journal of Mathematical Biology·Marcus J GroteIvan Martin
Nov 13, 2007·Diabetologia·G ParnaudP A Halban
Nov 21, 2007·Advanced Drug Delivery Reviews·Shimon Efrat
Jan 24, 2009·Annals of the New York Academy of Sciences·Darren J MichaelRobert H Chow
Mar 18, 2013·Journal of Diabetes Investigation·Kohtaro Minami, Susumu Seino
Sep 22, 2011·Islets·Vijayalakshmi VenkatesanRamesh R Bhonde
Jan 15, 2013·Cell Metabolism·Pablo HelgueraJorge Busciglio
May 7, 2003·Gene Therapy·R BottinoN Giannoukakis

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