Ex vivo expansion of umbilical cord blood CD34 cells in a closed system: a multicentric study

Vox Sanguinis
G AstoriS Querol

Abstract

The Dideco 'Pluricell System' is a CE-marked medical device allowing haematopoietic stem cell (HSC) expansion. It comprises a kit of cGMP cytokines and reagents, a closed-cell expansion chamber and a cell-washing set. We tested the system in a multicentric study by expanding CD34(+) cells from eight fresh umbilical cord blood (UCB) samples. During culture, the mean nucleated cell (NC) count, the mean CD34(+) cell count, fold expansion, viability and apoptosis were measured. Clonogenic assays and immunophenotypical characterization were performed on days 0, 7 and 12. On the expanded cellular product, in three cases cell genotyping, endotoxin level and mycoplasma detection (by polymerase chain reaction) were performed. The mean CD34(+) cell expansion on days 7 and 12 was sevenfold and 12-fold respectively and the mean NC expansion was 69-fold and 180-fold. The mean NC viability on day 12 was 96.9% (94.4-99.1). After 12 days, granulocyte-macrophage colony-forming units (GM-CFU) showed a 20-fold increase: a slight increase in CD34(+) cell apoptosis was observed during culture. In all of three cases neither chromosomal alterations nor mycoplasma contamination was detected. No significant endotoxin levels were detected after expansio...Continue Reading

References

Jun 1, 1996·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M J AlcornI M Franklin
Jul 17, 2001·Journal of Hematotherapy & Stem Cell Research·L Douay
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Citations

Apr 10, 2007·Annals of Biomedical Engineering·Xiubo FanZhanfeng Cui
Apr 1, 2009·Applied Biochemistry and Biotechnology·Mei ZhaoWei Han
Feb 16, 2008·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·J J NietfeldMary M Horowitz

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