PMID: 7540792Apr 1, 1995Paper

Ex vivo expansion of umbilical cord blood hematopoietic progenitor cells by combinations of cytokines

Acta Paediatrica Japonica; Overseas Edition
M UrashimaC Kaihara

Abstract

Ex vivo expansion of hematopoietic progenitor cells in the umbilical cord blood mononuclear cells (CB-MNC) was investigated in liquid culture system with various combinations of cytokines (stem cell factor [SCF], interleukin [IL]-3, IL-6, granulocyte-colony stimulating factor [G-CSF], erythropoietin [EPO], and interferon [INF]-gamma). Non-lineage-committed hematopoietic progenitor cells and lineage committed hematopoietic progenitor cells were represented as CD34+CD38- and CD34+CD38+ subpopulations, respectively. Although absolute CD34+CD38- cell numbers decreased even in the presence of multicytokines, the combinations of SCF plus IL-6 and SCF plus IL-3 plus IL-6 plus IFN-gamma were significantly effective in maintaining CD34+CD38- cells than the other combinations (P < 0.05). After 4 weeks of culture. CD34+CD38- cells disappeared in all combinations of cytokines. Absolute CD34+CD38+ cell numbers increased in the presence of cytokines. Maximal expansion of CD34+CD38+ cells were observed in the combinations of SCF plus IL-3 plus IL-6 plus EPO (19.8 +/- 3.3-fold) and SCF plus IL-3 plus IL-6 plus G-CSF (18.3 +/- 2.6). The combination of SCF plus IL-3 plus IL-6 was also effective to expand CD34+CD38- cells (15.8 +/- 3.9). However,...Continue Reading

References

Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H E BroxmeyerE A Boyse
Aug 1, 1993·Acta Paediatrica Japonica; Overseas Edition·M UrashimaK Maekawa

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Citations

Aug 5, 2009·Stem Cell Reviews and Reports·Marcus JägerRüdiger Krauspe
Jul 29, 1998·Leukemia Research·H DimitriouM Kalmanti
Jan 1, 1997·Pediatric Hematology and Oncology·D Schuler
Nov 8, 2014·Stem Cells Translational Medicine·Javier MunozElizabeth J Shpall

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