Ex vivo generated natural killer cells acquire typical natural killer receptors and display a cytotoxic gene expression profile similar to peripheral blood natural killer cells.

Stem Cells and Development
Dorit LehmannErhard Hofer

Abstract

Ex vivo differentiation systems of natural killer (NK) cells from CD34+ hematopoietic stem cells are of potential importance for adjuvant immunotherapy of cancer. Here, we analyzed ex vivo differentiation of NK cells from cord blood-derived CD34+ stem cells by gene expression profiling, real-time RT-PCR, flow cytometry, and functional analysis. Additionally, we compared the identified characteristics to peripheral blood (PB) CD56(bright) and CD56(dim) NK cells. The data show sequential expression of CD56 and the CD94 and NKG2 receptor chains during ex vivo NK cell development, resulting finally in the expression of a range of genes with partial characteristics of CD56(bright) and CD56(dim) NK cells from PB. Expression of characteristic NK cell receptors and cytotoxic genes was mainly found within the predominant ex vivo generated population of NKG2A+ NK cells, indicating the importance of NKG2A expression during NK cell differentiation and maturation. Furthermore, despite distinct phenotypic characteristics, the detailed analysis of cytolytic genes expressed within the ex vivo differentiated NK cells revealed a pattern close to CD56(dim) NK cells. In line with this finding, ex vivo generated NK cells displayed potent cytotoxici...Continue Reading

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Citations

Apr 18, 2013·European Journal of Immunology·Frank MomburgAdelheid Cerwenka
Sep 13, 2015·Journal of Experimental & Clinical Cancer Research : CR·Alessandra PicardiPatrizio Giacomini
Apr 30, 2015·Cancer Immunology, Immunotherapy : CII·Subhashis SarkarLotte Wieten
May 12, 2020·Journal of Leukocyte Biology·Barclay J LeeEmily M Mace
May 31, 2017·Frontiers in Immunology·Mirte PostErhard Hofer
May 8, 2021·Clinical & Translational Immunology·May DaherKatayoun Rezvani

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Methods Mentioned

BETA
density-gradient centrifugation
flow cytometry
FCS
fluorescence-activated cell sorting
FACS
flow
dot blots
PMA

Software Mentioned

Primer
BLAST
StepOne

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