PMID: 11328306May 1, 2001Paper

Ex vivo generation of human cytomegalovirus-specific cytotoxic T cells by peptide-pulsed dendritic cells

British Journal of Haematology
Annette KleihauerHermann Einsele

Abstract

Adoptive transfer of donor-derived human cytomegalovirus (HCMV)-specific T-cell clones can restore protective immunity after stem cell transplantation. Ex vivo induction of HCMV-specific T cells using HCMV-infected fibroblasts as stimulator cells confines this approach to HCMV-seropositive donors and requires the presence of infectious virus during the stimulation procedure. In this study, we describe a potential alternative strategy to generate HCMV-specific T cells ex vivo for adoptive immunotherapy. Generation of HCMV-specific cytotoxic T lymphocytes (CTLs) ex vivo was investigated using peptide-pulsed dendritic cells as antigen-presenting cells. HCMV-specific T cells were generated and sufficiently expanded for adoptive immunotherapy in 6 out of 14 HCMV-seropositive and 2 out of 11 HCMV-seronegative donors. The CTLs recognized HCMV-infected autologous fibroblasts. No lysis was observed with either non-infected autologous or HLA-mismatched infected fibroblasts. Staining with tetrameric HLA/peptide complexes revealed significant enrichment for peptide-specific T cells of up to 28% and > 90% of CD8(+) T cells after three and five specific stimulations respectively. In addition, the expansion rates indicated that ex vivo genera...Continue Reading

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Citations

Oct 15, 2003·Blood Reviews·Maher K GandhiAndrew J Carmichael
Nov 26, 2008·Expert Reviews in Molecular Medicine·John Sinclair
Jul 9, 2002·British Journal of Haematology·Per Ljungman
Oct 2, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John A Zaia
Mar 29, 2003·Journal of Hematotherapy & Stem Cell Research·Aaron E FosterKenneth F Bradstock
Oct 24, 2002·Current Opinion in Hematology·Hermann Einsele, Holger Hebart
Sep 28, 2007·Current Opinion in Hematology·Alana A Kennedy-Nasser, Malcolm K Brenner
Aug 25, 2012·Current Opinion in Hematology·Giacomo OliveiraChiara Bonini
Jun 14, 2008·Transplantation·Mario G Solari, Angus W Thomson
Oct 6, 2009·Current Opinion in Immunology·Giuseppina Li PiraHermann Einsele
Sep 14, 2007·Blood Cells, Molecules & Diseases·Hermann EinseleGötz Ulrich Grigoleit
Nov 10, 2006·British Journal of Haematology·Ilenia ChatziandreouH Bobby Gaspar
Aug 19, 2015·Transplant International : Official Journal of the European Society for Organ Transplantation·Andy Roemhild, Petra Reinke
Nov 30, 2004·The Lancet Infectious Diseases·Maher K Gandhi, Rajiv Khanna
Jun 3, 2004·Human Immunology·Hermann Einsele, Holger Hebart
Oct 6, 2007·Cytotherapy·M KappGu Grigoleit
Jul 31, 2013·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Cinja SukdolakBritta Eiz-Vesper
Oct 27, 2004·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Aaron E FosterDavid J Gottlieb
Feb 21, 2004·Journal of Neuroimmunology·Antonella FacchinettiGiovanni Biasi
May 13, 2017·Nature Reviews. Neurology·Michael WellerJohn H Sampson
Oct 12, 2010·Current Opinion in Organ Transplantation·Tan S MuiGötz U Grigoleit
Mar 15, 2006·Journal of Immunotherapy·Glenn A Van den BoschViggo F I Van Tendeloo
Oct 30, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrea SchubAndreas Moosmann

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