Ex vivo identification and characterization of a population of CD13(high) CD105(+) CD45(-) mesenchymal stem cells in human bone marrow

Stem Cell Research & Therapy
Carmen MuñizAlberto Orfao

Abstract

Mesenchymal stem cells (MSCs) are multipotent cells capable of self-renewal and multilineage differentiation. Their multipotential capacity and immunomodulatory properties have led to an increasing interest in their biological properties and therapeutic applications. Currently, the definition of MSCs relies on a combination of phenotypic, morphological and functional characteristics which are typically evaluated upon in vitro expansion, a process that may ultimately lead to modulation of the immunophenotypic, functional and/or genetic features of these cells. Therefore, at present there is great interest in providing markers and phenotypes for direct in vivo and ex vivo identification and isolation of MSCs. Multiparameter flow cytometry immunophenotypic studies were performed on 65 bone marrow (BM) samples for characterization of CD13(high) CD105(+) CD45(-) cells. Isolation and expansion of these cells was performed in a subset of samples in parallel to the expansion of MSCs from mononuclear cells following currently established procedures. The protein expression profile of these cells was further assessed on (paired) primary and in vitro expanded BM MSCs, and their adipogenic, chondrogenic and osteogenic differentiation potent...Continue Reading

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Citations

Aug 17, 2016·Journal of Biotechnology·António M de SoureJoaquim M S Cabral
Apr 14, 2017·American Journal of Hematology·José Antonio García-VelaMiguel Angel Alvarez Juarez
Oct 31, 2018·Blood Advances·Javier I Muñoz-GonzálezAndrés C García-Montero
Sep 14, 2018·Stem Cells International·Ross E B FitzsimmonsCraig A Simmons
May 29, 2021·Developmental Biology·Jorge B AquinoLaura A Montaldo

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Methods Mentioned

BETA
flow cytometry
density gradient centrifugation
fluorescence-activated cell sorter

Software Mentioned

SPSS
Infinicyt
FACSDiva

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