Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1

Journal of Visualized Experiments : JoVE
Elena RahnDagmar Knebel-Mörsdorf

Abstract

To enter its human host, herpes simplex virus type 1 (HSV-1) must overcome the barrier of mucosal surfaces, skin, or cornea. HSV-1 targets keratinocytes during initial entry and establishes a primary infection in the epithelium, which is followed by latent infection of neurons. After reactivation, viruses can become evident at mucocutaneous sites that appear as skin vesicles or mucosal ulcers. How HSV-1 invades skin or mucosa and reaches its receptors is poorly understood. To investigate the invasion route of HSV-1 into epidermal tissue at the cellular level, we established an ex vivo infection model of murine epidermis, which represents the site of primary and recurrent infection in skin. The assay includes the preparation of murine skin. The epidermis is separated from the dermis by dispase II treatment. After floating the epidermal sheets on virus-containing medium, the tissue is fixed and infection can be visualized at various times postinfection by staining infected cells with an antibody against the HSV-1 immediate early protein ICP0. ICP0-expressing cells can be observed in the basal keratinocyte layer already at 1.5 hr postinfection. With longer infection times, infected cells are detected in suprabasal layers, indicati...Continue Reading

Citations

Sep 8, 2017·Journal of Virology·Katharina ThierDagmar Knebel-Mörsdorf
Mar 23, 2017·Scientific Reports·Win Kyaw PhyuKum Thong Wong
Jul 29, 2020·EMBO Molecular Medicine·Luigi MaddalunoSabine Werner

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