Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease

Journal of Visualized Experiments : JoVE
Sean W MateerSimon Keely

Abstract

The epithelial barrier is the first innate defense of the gastrointestinal tract and selectively regulates transport from the lumen to the underlying tissue compartments, restricting the transport of smaller molecules across the epithelium and almost completely prohibiting epithelial macromolecular transport. This selectivity is determined by the mucous gel layer, which limits the transport of lipophilic molecules and both the apical receptors and tight junctional protein complexes of the epithelium. In vitro cell culture models of the epithelium are convenient, but as a model, they lack the complexity of interactions between the microbiota, mucous-gel, epithelium and immune system. On the other hand, in vivo assessment of intestinal absorption or permeability may be performed, but these assays measure overall gastrointestinal absorption, with no indication of site specificity. Ex vivo permeability assays using "intestinal sacs" are a rapid and sensitive method of measuring either overall intestinal integrity or comparative transport of a specific molecule, with the added advantage of intestinal site specificity. Here we describe the preparation of intestinal sacs for permeability studies and the calculation of the apparent per...Continue Reading

Citations

Apr 24, 2020·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Simon Keely, Nicholas J Talley
Sep 24, 2020·Oxidative Medicine and Cellular Longevity·Apor Veres-SzékelyÁdám Vannay
Jan 31, 2019·Frontiers in Physiology·Marianela González-GonzálezMarcela Julio-Pieper
Dec 29, 2020·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Caroline TwarogDavid J Brayden
Mar 21, 2017·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·John P GleesonSinéad M Ryan
May 18, 2021·Chemical Science·Farah BenyettouAli Trabolsi
Jul 18, 2021·Scientific Reports·Rita LippaiÁdám Vannay

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