Ex vivo liposome-mediated gene transfer to lung isografts

The Journal of Thoracic and Cardiovascular Surgery
C H BoasquevisqueG A Patterson

Abstract

Gene therapy is a promising strategy to modify ischemia-reperfusion injury and rejection after transplantation. We evaluated variables that may affect ex vivo gene transfer to rat lung isografts. Left lungs were harvested and perfused via the pulmonary vein with chloramphenicol acetyltransferase complementary deoxyribonucleic acid complexed with cationic liposomes. Several variables were examined: (1) Influence of temperature: In group I (n = 4), grafts were stored for 4 hours at 23 degrees C and transplanted. Chloramphenicol acetyltransferase activity was assessed on postoperative day 2. In groups II and III (n = 4), grafts were stored at 10 degrees and 4 degrees C, respectively. Arterial oxygen tension and inflammatory infiltrate were also determined. (2) Influence of storage time: Grafts were preserved at 10 degrees C for 1, 2, 3, 4 (n = 4), and 10 hours (n = 5). chloramphenicol acetyltransferase activity was assessed on postoperative day 2. (3) Rapidity and duration of transgene expression: Grafts were preserved at 10 degrees C for 1 hour and then transplanted. Chloramphenicol acetyltransferase activity was assessed 2, 4, 6, 12, and 24 hours and 2, 7, 14, 21, and 28 days after implantation. Chloramphenicol acetyltransferase...Continue Reading

Citations

Oct 13, 1998·The Annals of Thoracic Surgery·R LeeG A Patterson
Feb 2, 2000·The Annals of Thoracic Surgery·B N MoraG A Patterson
Jul 4, 2001·The Journal of Thoracic and Cardiovascular Surgery·H ItanoG A Patterson
Jun 4, 1999·The Annals of Thoracic Surgery·M HiratsukaG A Patterson
May 2, 2000·The Journal of Thoracic and Cardiovascular Surgery·B N MoraG A Patterson
Dec 31, 1998·The Journal of Thoracic and Cardiovascular Surgery·C H BoasquevisqueG A Patterson

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