Ex vivo magnetofection with magnetic nanoparticles: a novel platform for nonviral tissue engineering

Artificial Organs
Shieh-Yueh YangHerng-Er Horng

Abstract

Several methods have been described to introduce DNA expression vectors into mammalian cells both in vitro and in vivo. Each system has benefits and limitations, and to date there is still no ideal method for gene transfer. In this study, we introduced a novel method of gene transfer by using Fe3O4 nanoparticles. The magnetic nanoparticles composed of Fe3O4, and the transfected genes used are Lac Z and enhanced green fluorescence protein gene (EGFG). Four different groups of preparations included in this study were homemade liposome-enveloped EGFP-DNA/Fe3O4, homemade liposome EGFP-DNA gene without magnetic Fe3O4 nanoparticles, lipofectamine 2000-enveloped EGFP-DNA, and EGFP-DNA gene only. Mice osteoblast and He99 lung cancer cell line were used as host cells for gene transfection. The time-dependent EGFP gene expression was monitored and analyzed. The results showed that the diameter of the complex was less than 100 nm. There was no cytotoxicity observed at any of the magnetic Fe3O4 nanoparticle concentrations tested. In the presence of magnetic field, the liposome-enveloped EGFP-DNA/Fe3O4 complex exhibited a much higher efficiency for transfecting EGFP-DNA into osteoblast cells under external magnetic fields. The gene can be t...Continue Reading

References

Jan 24, 1991·Nature·P L Felgner, G Rhodes
Jan 26, 1989·Nature·P L Felgner, G M Ringold
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R W MaloneI M Verma
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P L FelgnerM Danielsen
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Jul 30, 1982·Biochemical and Biophysical Research Communications·T K Wong, E Neumann
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·A R ThierryL C Mahan
Aug 1, 1995·Trends in Genetics : TIG·D C Prasher
Jan 1, 1997·Journal of Magnetic Resonance Imaging : JMRI·D G Mitchell
Apr 3, 1997·Biochimica Et Biophysica Acta·S J EastmanR K Scheule
Dec 1, 1995·Molecular Medicine Today·W H Günzburg, B Salmons
Jul 24, 1998·Biochemical and Biophysical Research Communications·K Sawai, D Meruelo
Sep 22, 1998·Nature Biotechnology·H Aihara, J Miyazaki
Nov 6, 1998·Pharmacology & Therapeutics·P D Robbins, S C Ghivizzani
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MirD Scherman
Jun 15, 1999·Analytical Chemistry·J A KoropchakS L Kaufman
Nov 5, 1999·Nature Medicine·T Kielian, W F Hickey
Apr 13, 2000·Journal of Drug Targeting·J M Benns, S W Kim
Jan 17, 2002·Bioconjugate Chemistry·Dagmar HögemannRalph Weissleder
Nov 27, 2002·Biochemistry·Veena VijayanathanT J Thomas
Mar 12, 2003·Advanced Drug Delivery Reviews·Jayanth Panyam, Vinod Labhasetwar
Apr 30, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Florian KrötzChristian Plank
Jun 24, 2003·Biological Chemistry·Christian PlankJoseph Rosenecker
Jul 26, 2003·Expert Opinion on Biological Therapy·Christian PlankFlorian Krötz
Oct 8, 2003·Journal of Vascular Research·Florian KrötzUlrich Pohl
Jan 13, 2004·Methods in Enzymology·William M Pardridge
Aug 5, 2004·The Journal of Gene Medicine·Stephanie HuthJoseph Rosenecker
May 6, 2005·Human Gene Therapy·Nicholas Muzyczka, Kenneth H Warrington
Jul 19, 2005·Current Medicinal Chemistry·B BellettiG Baldassarre

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Citations

Oct 27, 2012·Annals of Biomedical Engineering·Adam J MellottMichael S Detamore
Feb 28, 2009·Artificial Organs·Paul S Malchesky
Jul 13, 2012·International Journal of Nanomedicine·Kai-Wen HuangHong-Chang Yang
Nov 15, 2011·Nanomedicine·Evgenya DelyaginaGustav Steinhoff
Mar 1, 2011·Radiology and Oncology·Sara Prijic, Gregor Sersa
Jul 13, 2011·International Journal of Molecular Sciences·Daisuke KamiMasashi Toyoda
Sep 26, 2013·Pharmaceutical Research·Ruijun XingXiaoyuan Chen
Jan 25, 2011·Biomaterials·Souvik BiswasMichael H Nantz
Nov 12, 2013·Critical Reviews in Oncology/hematology·Nian-feng SunSan-yuan Hu
Feb 4, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Mahasweta DasSubhra Mohapatra
Apr 25, 2012·Journal of Biomedical Materials Research. Part a·Jui-Tse ChenRavin Narain
Jul 15, 2015·Nanoscale Research Letters·Jen-Jie ChiehMing-Hsien Chiang
Jan 22, 2009·British Journal of Pharmacology·Julien Villemejane, Lluis M Mir
Dec 17, 2020·Frontiers in Neuroscience·José HurstSven Schnichels

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