Ex vivo microperfusion system of the adipose organ: a new approach to studying the mobilization of adipose cell populations

International Journal of Obesity : Journal of the International Association for the Study of Obesity
Marta Gil-OrtegaC Sengenès

Abstract

Adipose tissue (AT) is a dynamic organ that expands and contracts rapidly. It is composed of adipocytes and of cell populations among which immune cells and mesenchymal progenitors known as adipose stromal cells (ASCs). The AT cell turnover has been extensively studied. Surprisingly it has only been viewed as the result of both cell proliferation/death and cell infiltration. Nevertheless, both immune cells and ASCs exhibit migration abilities; therefore their egress from AT in response to physiological/pathophysiological stimuli has to be considered. To do so, the aim of the present work was to develop a model allowing the study of cell release from the adipose organ. Mesenteric (Mes) ATs were isolated from 9-week-old C57BL/6 male mice and were catheterized via the superior mesenteric artery and were perfused with a saline solution. After an equilibration period, the mesenteric fat pad was perfused with CXCL12 (stromal-derived factor-1, SDF-1) or sphingosine 1-phosphate (S1P) to trigger cell mobilization and perfusates were collected every 30 min for subsequent flow cytometry analyses. We report here that CXCL12 induces the specific release of ASCs from MesAT thus demonstrating that ASCs are specifically mobilized from fat depo...Continue Reading

References

Jul 18, 2002·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·D B HausmanR J Martin
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Jun 15, 2005·FEBS Letters·S Caspar-BauguilL Pénicaud
Apr 8, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Beatriz GálvezMaria S Fernández Alfonso
Apr 26, 2006·The Journal of Experimental Medicine·Aarif Y KhakooToren Finkel
Dec 5, 2006·The Journal of Clinical Investigation·Joseph T CrossnoDwight J Klemm
May 15, 2007·Circulation Research·Jeffrey M GimbleBruce A Bunnell
Jul 10, 2007·Cancer Research·Lucia KucerovaCestmir Altaner
Jan 30, 2008·Circulation·V BourlierA Bouloumié
Apr 5, 2008·Stem Cells·Gwendal Lazennec, Christian Jorgensen
May 6, 2008·Nature·Kirsty L SpaldingPeter Arner
Aug 7, 2008·The Journal of Clinical Endocrinology and Metabolism·Marie MaumusAnne Bouloumié
Jun 23, 2009·Nature Medicine·Yorihiro NishimuraHiroyuki Shimizu
Mar 17, 2011·PloS One·Alessandra RigamontiChad A Cowan
May 25, 2011·World Journal of Stem Cells·Yan ZhangMikhail G Kolonin
May 28, 2011·Methods in Molecular Biology·Claire MagnonPaul S Frenette
Jul 8, 2011·Stem Cells and Development·Marijke W MaijenburgCarlijn Voermans
Aug 2, 2011·Trends in Immunology·Irina B MazoUlrich H von Andrian
Feb 1, 2012·PloS One·Kazushiro FujiwaraKazuaki Yoshikawa
Jul 10, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Blanca OñateLina Badimon
Sep 29, 2012·Nature Reviews. Immunology·Jean-Philippe GirardReinhold Förster

❮ Previous
Next ❯

Citations

Dec 20, 2019·Angiogenesis·M di SommaS Mitola
Jan 26, 2021·Frontiers in Cell and Developmental Biology·Amandine GirousseCoralie Sengenès

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.