Ex vivo pharmacology of surgical samples of the uterosacral ligament. Part I: Effects of carbachol and oxytocin on smooth muscle

Neurourology and Urodynamics
Ulrich DrewsChristl Reisenauer

Abstract

In a previous study we observed impaired smooth muscle in the uterosacral ligament (USL) of patients with pelvic organ prolapse. The aims of the study were to describe the method of the novel microperfusion system and to determine normal function and pharmacology of smooth muscle in the USL. Samples from the USL were obtained during hysterectomy for benign reasons. Small stretches of connective tissue were mounted in a perfusion chamber under the stereomicroscope. Isotonic contractions of smooth muscle were monitored by digital time-lapse video and quantified by image processing. Constant perfusion with carbachol elicited tonic and pulse stimulation with carbachol and oxytocin rhythmic contractions of smooth muscle in the ground reticulum. Under constant perfusion with relaxin the tonic contraction after carbachol was abolished. With the novel microperfusion system, isotonic contractions of smooth muscle in the USL can be recorded and quantified in the tissue microenvironment on the microscopic level. The USL smooth muscle is cholinergic, stimulated by oxytocin and modulated by relaxin.

References

Mar 3, 2004·Obstetrics and Gynecology·Wolfgang H UmekJohn O L DeLancey
Jan 1, 1950·American Journal of Obstetrics and Gynecology·R M CAMPBELL
Jul 14, 2005·Clinical Obstetrics and Gynecology·Joseph I SchafferMuriel K Boreham
Dec 12, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Takio KitazawaTetsuro Taneike
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Mar 14, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Joanna E GullamAnatoly Shmygol
Jul 16, 2010·CNS Neuroscience & Therapeutics·Cedric VieroGovindan Dayanithi
Jul 16, 2010·Endocrine Reviews·Felice PetragliaJohn R G Challis

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