PMID: 9160095May 1, 1997Paper

Ex vivo PKH26-labelling of lymphocytes for studies of cell migration in vivo

Scandinavian Journal of Immunology
C JohnssonT H Tötterman

Abstract

A prerequisite for studies of cell migration is that the cells of interest can be appropriately labelled and subsequently easily traced. The use of radioisotopes or fluorescent substances that bind covalently to the cell surface, e.g. fluorescein isothiocyanate (FITC) or rhodamine isothiocyanate (RITC), have limitations such as rapid loss of the labelling, toxicity and interference with cell surface molecules. In the present work the authors labelled rat spleen lymphocytes with the fluorescent labelling molecule PKH26, which is incorporated into the lipid bilayer of cytoplasmic membranes. The labelled lymphocytes were injected intravenously into syngeneic recipients and 2 or 6 days later the lymphocytes were detected in various organs by using flow cytometry and fluorescence microscopy. As could be expected, the lymphocytes homed to lymphoid tissues, preferably the spleen, and no labelled cells were found in non-lymphoid organs such as the heart and the kidney. Membrane labelling proved to be intense, uniform and stable and PKH26 positive cells were easily detectable in fractions less than 0.2% in peripheral blood and the various tissues after 6 days of in vivo circulation. Thus, the PKH26 dye appears to be suitable for labelli...Continue Reading

Citations

Jun 10, 2003·Plastic and Reconstructive Surgery·Bernd Rieck, Sabine Schlaak
Aug 30, 2014·Aesthetic Surgery Journal·Maciej NowackiRafał Czajkowski
Feb 18, 2017·International Journal of Molecular Sciences·Pooi Ling MokKong Yong Then
Sep 8, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Martijn A NolteReina E Mebius
Nov 26, 1999·Immunology and Cell Biology·C R Parish
Jan 5, 2002·In Vitro Cellular & Developmental Biology. Animal·C RousselleX Ronot
Aug 29, 2006·World Journal of Gastroenterology : WJG·Yu-Tao ZhanRan Fei
Dec 18, 2001·European Journal of Immunology·L Santos-ArgumedoL Flores-Romo

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