Ex vivo real-time monitoring of volatile metabolites resulting from nasal odorant metabolism

Scientific Reports
Aline Robert-HazotteJean-Marie Heydel

Abstract

Odorant-metabolizing enzymes are critically involved in the clearance of odorant molecules from the environment of the nasal neuro-olfactory tissue to maintain the sensitivity of olfactory detection. Odorant metabolism may also generate metabolites in situ, the characterization and function of which in olfaction remain largely unknown. Here, we engineered and validated an ex vivo method to measure odorant metabolism in real-time. Glassware containing an explant of rat olfactory mucosa was continuously flushed with an odorant flow and was coupled to a proton transfer reaction-mass spectrometer for volatile compound analysis. Focusing on carboxylic esters and diketone odorants, we recorded the metabolic uptake of odorants by the mucosa, concomitantly with the release of volatile odorant metabolites in the headspace. These results significantly change the picture of real-time in situ odorant metabolism and represent a new step forward in the investigation of the function of odorant metabolites in the peripheral olfactory process. Our method allows the systematic identification of odorant metabolites using a validated animal model and permits the screening of olfactory endogenously produced chemosensory molecules.

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Citations

Mar 1, 2019·Scientific Reports·Aline Robert-HazotteJean-Marie Heydel
Jul 25, 2019·PloS One·Jean-Marie HeydelFabrice Neiers
Jun 18, 2019·Drug Metabolism Reviews·Jean-Marie HeydelFabrice Neiers
Aug 22, 2020·Chemical Senses·Mathieu SchwartzFabrice Neiers
Jun 30, 2019·Chemical Senses·Chiori IjichiKazushige Touhara
Apr 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·María Perez-JiménezMaría Angeles Pozo-Bayón
Jan 8, 2021·Cell and Tissue Research·Smija M KurianLuis R Saraiva
Oct 16, 2020·Chemistry : a European Journal·Tunga SalthammerWolf-Ulrich Palm
Oct 13, 2021·International Forum of Allergy & Rhinology·Chiori IjichiKenji Kondo
Oct 16, 2021·Comprehensive Reviews in Food Science and Food Safety·Mathieu SchwartzFrancis Canon

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