Ex vivo sentinel lymph node mapping in colon cancer: improving the accuracy of pathologic staging?

American Journal of Surgery
Jenni SmithP Terry Phang

Abstract

A subset of patients with colon cancer staged by conventional methods have occult micrometastases and do not receive adjuvant chemotherapy. Sentinel lymph node (SLN) mapping and staining by immunohistochemistry is a technique that may identify such occult micrometastases, thereby upstaging patients with positive findings. The purpose of this study was to determine whether ex vivo SLN mapping in colon cancer could be applied successfully to patients at our institution. Seventeen patients with intraperitoneal colon tumors undergoing resection were studied prospectively. SLNs were identified as the first blue stained node(s) after ex vivo peritumoral injection of isosulfan blue dye. Additional lymph nodes were harvested and processed in accordance with standard pathologic evaluation for colon cancer. All nodes were examined after routine hematoxylin and eosin (H&E) staining. SLNs that were negative on H&E were analyzed further by multilevel sectioning and immunohistochemistry staining using anticytokeratin monoclonal antibody. Of the 17 study patients, SLNs were identified in 16 (94%) cases. The SLN was the only positive node in 3 patients. An identified SLN was positive (by H&E) in all patients with associated positive non-SLN no...Continue Reading

References

Apr 1, 1991·Cancer·A M CohenE R Sigurdson
May 20, 1998·Seminars in Surgical Oncology·M C KelleyD L Morton
Jul 23, 1998·The New England Journal of Medicine·G J LiefersR A Tollenaar
Apr 24, 1999·The British Journal of Surgery·J J JoostenT J Ruers
Aug 7, 1999·Journal of the American College of Surgeons·S S BassD S Reintgen
Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J H WongT S Namiki
Apr 6, 2001·Diseases of the Colon and Rectum·A E MerrieJ L Mccall
Apr 17, 2001·Annals of Surgery·J H WongT Namiki
Jun 8, 2001·Diseases of the Colon and Rectum·S EsserS Arcona
Apr 23, 2002·Journal of Surgical Oncology·T L FitzgeraldA J Smith
May 23, 2002·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Thomas F WoodAnton J Bilchik
Apr 15, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Carolyn C Compton
May 14, 2003·Archives of Pathology & Laboratory Medicine·Roderick R TurnerAnton J Bilchik
Feb 5, 2005·Diseases of the Colon and Rectum·Stephen W BellEmmanuel Tiret
Jul 26, 2005·Journal of the American College of Surgeons·Alexander StojadinovicGeorge E Peoples

❮ Previous
Next ❯

Citations

May 8, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Bruno MärklHans M Arnholdt
Jan 8, 2010·Annals of Surgery·Aviram NissanAlexander Stojadinovic
Nov 19, 2010·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·E B RivetJ W Fleshman
Mar 25, 2010·Annals of Surgical Oncology·Bruno MärklTherese Gannon Kerwel
May 31, 2012·Annals of Surgical Oncology·Edwin S van der ZaagChristianne J Buskens
May 9, 2013·World Journal of Surgery·Jamie Murphy, Tonia Young-Fadok
May 10, 2011·The Lancet Oncology·Martijn Hgm van der PasWilhelmus J H J Meijerink
Feb 19, 2010·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·E S van der ZaagC J Buskens
Oct 16, 2012·Seminars in Diagnostic Pathology·Jinru ShiaN Volkan Adsay
Dec 24, 2018·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·L TangD Kong
Dec 20, 2007·American Journal of Clinical Pathology·Jeremy R JassUNKNOWN Association of Directors of Anatomic and Surgical Pathology
Feb 23, 2010·Diseases of the Colon and Rectum·Kelly R FinanJames W Fleshman
Aug 11, 2017·Surgical Laparoscopy, Endoscopy & Percutaneous Techniques·Aleix Martínez-PérezNicola de'Angelis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.